Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20741 | 3' | -57 | NC_004688.1 | + | 44092 | 0.69 | 0.633932 |
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Target: 5'- -gUUCCCCGUcCGGguugaGGAUGucguCGCGCCa -3' miRNA: 3'- uaGAGGGGUA-GCC-----UCUGCuu--GCGCGG- -5' |
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| 20741 | 3' | -57 | NC_004688.1 | + | 90990 | 0.7 | 0.60295 |
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Target: 5'- cGUCUaCCCgAUCGGAGguGCGGAccucgugguCGCGCUc -3' miRNA: 3'- -UAGA-GGGgUAGCCUC--UGCUU---------GCGCGG- -5' |
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| 20741 | 3' | -57 | NC_004688.1 | + | 11623 | 0.7 | 0.581368 |
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Target: 5'- gAUCUCCacucgagCCugaucuUCGGGGGCGAGCGCcucaaacugcgGCCa -3' miRNA: 3'- -UAGAGG-------GGu-----AGCCUCUGCUUGCG-----------CGG- -5' |
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| 20741 | 3' | -57 | NC_004688.1 | + | 36013 | 0.71 | 0.511927 |
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Target: 5'- ---aCCCCAacUCGGGGGCGAGggUGaCGCCa -3' miRNA: 3'- uagaGGGGU--AGCCUCUGCUU--GC-GCGG- -5' |
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| 20741 | 3' | -57 | NC_004688.1 | + | 17357 | 0.71 | 0.511927 |
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Target: 5'- aAUCUCCgcucgcccuCCA-CGGGGGCGGagacaacgaaguGCGCGCCc -3' miRNA: 3'- -UAGAGG---------GGUaGCCUCUGCU------------UGCGCGG- -5' |
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| 20741 | 3' | -57 | NC_004688.1 | + | 98663 | 0.71 | 0.50213 |
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Target: 5'- -aCgcgCCCCGagaGGucuGGACGGACGCGCCc -3' miRNA: 3'- uaGa--GGGGUag-CC---UCUGCUUGCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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