Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20742 | 3' | -49.5 | NC_004688.1 | + | 95284 | 0.67 | 0.983766 |
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Target: 5'- -cUGGAcCAGCGAauGACCGGCA--GAGg -3' miRNA: 3'- ccAUCUcGUCGCU--UUGGUCGUaaCUC- -5' |
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| 20742 | 3' | -49.5 | NC_004688.1 | + | 101100 | 0.67 | 0.979379 |
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Target: 5'- aGGUGGAGCuGCGGGcGCUGGUg--GAGu -3' miRNA: 3'- -CCAUCUCGuCGCUU-UGGUCGuaaCUC- -5' |
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| 20742 | 3' | -49.5 | NC_004688.1 | + | 56972 | 0.67 | 0.979379 |
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Target: 5'- gGGgcGGGCAGgGuucguuAAGCCAGCucaUGAGc -3' miRNA: 3'- -CCauCUCGUCgC------UUUGGUCGua-ACUC- -5' |
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| 20742 | 3' | -49.5 | NC_004688.1 | + | 20042 | 0.68 | 0.964648 |
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Target: 5'- -cUGGGGCGGCagcuuccggaacGAAGCCGGCGggGAu -3' miRNA: 3'- ccAUCUCGUCG------------CUUUGGUCGUaaCUc -5' |
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| 20742 | 3' | -49.5 | NC_004688.1 | + | 18310 | 0.69 | 0.94847 |
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Target: 5'- uGGUAGAGgAcCGAcuCCAGCAU-GGGg -3' miRNA: 3'- -CCAUCUCgUcGCUuuGGUCGUAaCUC- -5' |
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| 20742 | 3' | -49.5 | NC_004688.1 | + | 104510 | 0.7 | 0.933539 |
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Target: 5'- gGGUGGAGCuggacAGCGGAcCCGGCGa---- -3' miRNA: 3'- -CCAUCUCG-----UCGCUUuGGUCGUaacuc -5' |
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| 20742 | 3' | -49.5 | NC_004688.1 | + | 40738 | 0.71 | 0.902475 |
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Target: 5'- cGGUGGAGgGGCGGgccucugAACCGGUg--GAGa -3' miRNA: 3'- -CCAUCUCgUCGCU-------UUGGUCGuaaCUC- -5' |
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| 20742 | 3' | -49.5 | NC_004688.1 | + | 98951 | 0.72 | 0.873994 |
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Target: 5'- ---cGGGCAauugaagguccuGCGGGACCAgGCAUUGAGa -3' miRNA: 3'- ccauCUCGU------------CGCUUUGGU-CGUAACUC- -5' |
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| 20742 | 3' | -49.5 | NC_004688.1 | + | 8308 | 0.76 | 0.636003 |
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Target: 5'- uGGUAG-GCGGUGAAACgGGCcgUGAc -3' miRNA: 3'- -CCAUCuCGUCGCUUUGgUCGuaACUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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