Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20744 | 3' | -58.4 | NC_004688.1 | + | 71687 | 0.66 | 0.765892 |
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Target: 5'- gCUUUaaaaagGGUGCCgUCguauuccGCGGGCUCCGGa -3' miRNA: 3'- aGGAGg-----CCAUGG-AGa------UGCCCGAGGUC- -5' |
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| 20744 | 3' | -58.4 | NC_004688.1 | + | 39133 | 0.66 | 0.727149 |
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Target: 5'- cCCUCCGGUACCgaccGCGGaGUUaCCu- -3' miRNA: 3'- aGGAGGCCAUGGaga-UGCC-CGA-GGuc -5' |
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| 20744 | 3' | -58.4 | NC_004688.1 | + | 94002 | 0.67 | 0.707246 |
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Target: 5'- aUCCcCCGccACCUCUACGcGGCagCGGa -3' miRNA: 3'- -AGGaGGCcaUGGAGAUGC-CCGagGUC- -5' |
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| 20744 | 3' | -58.4 | NC_004688.1 | + | 20825 | 0.67 | 0.666748 |
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Target: 5'- cCCUCgCGGUGCCacagaGCGGGCUugCCGa -3' miRNA: 3'- aGGAG-GCCAUGGaga--UGCCCGA--GGUc -5' |
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| 20744 | 3' | -58.4 | NC_004688.1 | + | 82044 | 0.69 | 0.554591 |
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Target: 5'- cUCUCCGGUuucACCcaguUCUugGGGCcgaauucgaucUCCGGg -3' miRNA: 3'- aGGAGGCCA---UGG----AGAugCCCG-----------AGGUC- -5' |
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| 20744 | 3' | -58.4 | NC_004688.1 | + | 87829 | 0.7 | 0.524744 |
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Target: 5'- uUCUUCgCGG-GCCUCUuugaGGGCUuCCAGg -3' miRNA: 3'- -AGGAG-GCCaUGGAGAug--CCCGA-GGUC- -5' |
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| 20744 | 3' | -58.4 | NC_004688.1 | + | 87302 | 0.71 | 0.461481 |
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Target: 5'- aUCCUCCGGUAUCgguUCgccccagcggauggaUAcgggauugccgcCGGGCUCCAGg -3' miRNA: 3'- -AGGAGGCCAUGG---AG---------------AU------------GCCCGAGGUC- -5' |
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| 20744 | 3' | -58.4 | NC_004688.1 | + | 106439 | 0.72 | 0.378927 |
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Target: 5'- cUCUCCGGUACCgaUGCGGaGUUCCGc -3' miRNA: 3'- aGGAGGCCAUGGagAUGCC-CGAGGUc -5' |
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| 20744 | 3' | -58.4 | NC_004688.1 | + | 60694 | 0.74 | 0.309718 |
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Target: 5'- gUCC-CagaGGUGCCUCUgGCGGGCUUCGa -3' miRNA: 3'- -AGGaGg--CCAUGGAGA-UGCCCGAGGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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