Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20747 | 5' | -55.8 | NC_004688.1 | + | 36813 | 0.66 | 0.818613 |
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Target: 5'- cAGcgCC-CACCCACCCGaagccGGUGGGc -3' miRNA: 3'- aUCa-GGaGUGGGUGGGUau---CUACCCa -5' |
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| 20747 | 5' | -55.8 | NC_004688.1 | + | 19266 | 0.66 | 0.818613 |
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Target: 5'- -cGUCCUCuccCCCGCCgAUGcGGUGGa- -3' miRNA: 3'- auCAGGAGu--GGGUGGgUAU-CUACCca -5' |
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| 20747 | 5' | -55.8 | NC_004688.1 | + | 70144 | 0.67 | 0.79948 |
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Target: 5'- aUAGUCCguggggaaugUCACCCGCCCGcggggaUAGucauuucucgacgGUGGGa -3' miRNA: 3'- -AUCAGG----------AGUGGGUGGGU------AUC-------------UACCCa -5' |
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| 20747 | 5' | -55.8 | NC_004688.1 | + | 66852 | 0.69 | 0.648574 |
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Target: 5'- cAGUCCUCGCCauCCCAgugaUGGAUGcGGa -3' miRNA: 3'- aUCAGGAGUGGguGGGU----AUCUAC-CCa -5' |
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| 20747 | 5' | -55.8 | NC_004688.1 | + | 90116 | 0.71 | 0.553705 |
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Target: 5'- aGGUCUUCGCCCuCCCAccagcGGGUGaGGUg -3' miRNA: 3'- aUCAGGAGUGGGuGGGUa----UCUAC-CCA- -5' |
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| 20747 | 5' | -55.8 | NC_004688.1 | + | 102097 | 0.75 | 0.337767 |
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Target: 5'- gGGUCCgacaacuacggccgaACCCAUCUAUGGGUGGGUg -3' miRNA: 3'- aUCAGGag-------------UGGGUGGGUAUCUACCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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