Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20749 | 5' | -56.1 | NC_004688.1 | + | 6361 | 0.66 | 0.844715 |
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Target: 5'- cCUCGUacucGUCGAgGAucuucuccuCGGUCUGGCGAUa -3' miRNA: 3'- -GAGCGa---CAGCUaCU---------GCCGGAUCGCUA- -5' |
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| 20749 | 5' | -56.1 | NC_004688.1 | + | 45091 | 0.66 | 0.827785 |
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Target: 5'- gCUCGUcGgUGGUGGCGGCCUgcuuAGCGu- -3' miRNA: 3'- -GAGCGaCaGCUACUGCCGGA----UCGCua -5' |
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| 20749 | 5' | -56.1 | NC_004688.1 | + | 28186 | 0.69 | 0.682007 |
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Target: 5'- -cCGCUG-CGcAUGACGGUguaCUGGCGGUc -3' miRNA: 3'- gaGCGACaGC-UACUGCCG---GAUCGCUA- -5' |
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| 20749 | 5' | -56.1 | NC_004688.1 | + | 54312 | 0.69 | 0.661107 |
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Target: 5'- aCUCGCcGUUGGUGAUGuGCCcgcGGCGGc -3' miRNA: 3'- -GAGCGaCAGCUACUGC-CGGa--UCGCUa -5' |
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| 20749 | 5' | -56.1 | NC_004688.1 | + | 44512 | 0.7 | 0.629595 |
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Target: 5'- --aGCcGUUGAUGAUGGCCUgcacaAGCGGa -3' miRNA: 3'- gagCGaCAGCUACUGCCGGA-----UCGCUa -5' |
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| 20749 | 5' | -56.1 | NC_004688.1 | + | 79022 | 0.7 | 0.619083 |
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Target: 5'- -aUGUUGUUGAUgGGCGGCCccgGGCGGa -3' miRNA: 3'- gaGCGACAGCUA-CUGCCGGa--UCGCUa -5' |
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| 20749 | 5' | -56.1 | NC_004688.1 | + | 13509 | 0.74 | 0.393571 |
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Target: 5'- -gCGCgacGUCGuUGACGGCCUcGGCGAUu -3' miRNA: 3'- gaGCGa--CAGCuACUGCCGGA-UCGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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