Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2075 | 5' | -61.4 | NC_001348.1 | + | 106004 | 0.67 | 0.534288 |
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Target: 5'- aCUCGcuGGCCcaaaGGUGCGCGAcGAugCAAa -3' miRNA: 3'- -GAGCcuCCGG----CCGCGCGCU-CUugGUU- -5' |
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| 2075 | 5' | -61.4 | NC_001348.1 | + | 124422 | 0.67 | 0.534288 |
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Target: 5'- gUCGGuGGCCccgGGUGUgcuGCGAGAccGCCGGg -3' miRNA: 3'- gAGCCuCCGG---CCGCG---CGCUCU--UGGUU- -5' |
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| 2075 | 5' | -61.4 | NC_001348.1 | + | 105370 | 0.67 | 0.534288 |
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Target: 5'- gUCGGuGGCCccgGGUGUgcuGCGAGAccGCCGGg -3' miRNA: 3'- gAGCCuCCGG---CCGCG---CGCUCU--UGGUU- -5' |
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| 2075 | 5' | -61.4 | NC_001348.1 | + | 123788 | 0.67 | 0.534288 |
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Target: 5'- aCUCGcuGGCCcaaaGGUGCGCGAcGAugCAAa -3' miRNA: 3'- -GAGCcuCCGG----CCGCGCGCU-CUugGUU- -5' |
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| 2075 | 5' | -61.4 | NC_001348.1 | + | 123994 | 0.71 | 0.337418 |
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Target: 5'- -cCGGAGGCCGGCGacguacccaccuggGCGAGGguguuuugccGCCAc -3' miRNA: 3'- gaGCCUCCGGCCGCg-------------CGCUCU----------UGGUu -5' |
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| 2075 | 5' | -61.4 | NC_001348.1 | + | 105798 | 0.71 | 0.337418 |
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Target: 5'- -cCGGAGGCCGGCGacguacccaccuggGCGAGGguguuuugccGCCAc -3' miRNA: 3'- gaGCCUCCGGCCGCg-------------CGCUCU----------UGGUu -5' |
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| 2075 | 5' | -61.4 | NC_001348.1 | + | 120983 | 1.05 | 0.001332 |
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Target: 5'- uCUCGGAGGCCGGCGCGCGAGAACCAAc -3' miRNA: 3'- -GAGCCUCCGGCCGCGCGCUCUUGGUU- -5' |
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| 2075 | 5' | -61.4 | NC_001348.1 | + | 108809 | 1.05 | 0.001332 |
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Target: 5'- uCUCGGAGGCCGGCGCGCGAGAACCAAc -3' miRNA: 3'- -GAGCCUCCGGCCGCGCGCUCUUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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