Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20750 | 5' | -52.3 | NC_004688.1 | + | 57924 | 0.7 | 0.849136 |
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Target: 5'- gGGGCugcUGGCCUguggcggUGUaGGCGAAGUcgucgguguccAGCg -3' miRNA: 3'- -CCCGu--ACCGGA-------ACAgCUGCUUCA-----------UCG- -5' |
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| 20750 | 5' | -52.3 | NC_004688.1 | + | 324 | 0.7 | 0.824277 |
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Target: 5'- cGGGCugauaguaGGCCUUGuuUCGGCaGGAGUcGCu -3' miRNA: 3'- -CCCGua------CCGGAAC--AGCUG-CUUCAuCG- -5' |
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| 20750 | 5' | -52.3 | NC_004688.1 | + | 7000 | 0.7 | 0.824277 |
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Target: 5'- cGGGCGUGGgCUUggcGUUGAUGgcGUgcuucAGCa -3' miRNA: 3'- -CCCGUACCgGAA---CAGCUGCuuCA-----UCG- -5' |
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| 20750 | 5' | -52.3 | NC_004688.1 | + | 11348 | 0.7 | 0.815324 |
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Target: 5'- cGGGC--GGCggUGaaGGCGAGGUAGCc -3' miRNA: 3'- -CCCGuaCCGgaACagCUGCUUCAUCG- -5' |
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| 20750 | 5' | -52.3 | NC_004688.1 | + | 108113 | 0.73 | 0.707086 |
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Target: 5'- uGGGCA-GGUCUggacuaccgCGACGAGGaUAGCg -3' miRNA: 3'- -CCCGUaCCGGAaca------GCUGCUUC-AUCG- -5' |
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| 20750 | 5' | -52.3 | NC_004688.1 | + | 19457 | 0.73 | 0.655359 |
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Target: 5'- cGGGCGcucuucacgcuugugGGCCUcGUCGGCGuAGUAGg -3' miRNA: 3'- -CCCGUa--------------CCGGAaCAGCUGCuUCAUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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