Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20751 | 3' | -55.4 | NC_004688.1 | + | 55829 | 0.66 | 0.867261 |
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Target: 5'- uGGUcgUGAUCGagGUGGGCG-CCGCGa -3' miRNA: 3'- uCCAcaAUUGGCa-CGCCUGCaGGCGC- -5' |
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| 20751 | 3' | -55.4 | NC_004688.1 | + | 104511 | 0.67 | 0.86268 |
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Target: 5'- uGGGUGgagcuggacagcgGACCcggcgacuucauGUcacccGCGGACGUCCGCa -3' miRNA: 3'- -UCCACaa-----------UUGG------------CA-----CGCCUGCAGGCGc -5' |
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| 20751 | 3' | -55.4 | NC_004688.1 | + | 63738 | 0.67 | 0.859583 |
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Target: 5'- gAGGUGaucgaucACCGUggaGCGGGCcUCCGCc -3' miRNA: 3'- -UCCACaau----UGGCA---CGCCUGcAGGCGc -5' |
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| 20751 | 3' | -55.4 | NC_004688.1 | + | 75588 | 0.68 | 0.800319 |
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Target: 5'- gGGGUGUcGGCCGccGCGGACuccUCCGg- -3' miRNA: 3'- -UCCACAaUUGGCa-CGCCUGc--AGGCgc -5' |
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| 20751 | 3' | -55.4 | NC_004688.1 | + | 65453 | 0.68 | 0.791158 |
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Target: 5'- cGGGUGgcccuCCGggaUGCGGACGuaUCCGUu -3' miRNA: 3'- -UCCACaauu-GGC---ACGCCUGC--AGGCGc -5' |
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| 20751 | 3' | -55.4 | NC_004688.1 | + | 44776 | 0.7 | 0.672421 |
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Target: 5'- aAGGUGcccGCCGcgGUGGugG-CCGCGa -3' miRNA: 3'- -UCCACaauUGGCa-CGCCugCaGGCGC- -5' |
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| 20751 | 3' | -55.4 | NC_004688.1 | + | 42926 | 0.71 | 0.630951 |
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Target: 5'- cGGGUGggGACCGUucuguccagcgaGCGGAcuCGcCCGUGa -3' miRNA: 3'- -UCCACaaUUGGCA------------CGCCU--GCaGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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