Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20752 | 5' | -55.8 | NC_004688.1 | + | 61797 | 0.66 | 0.848716 |
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Target: 5'- -gCUGcGgGUCCAGCGGCc-GUAGUg -3' miRNA: 3'- aaGACcUgCAGGUCGCCGaaCAUCGg -5' |
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| 20752 | 5' | -55.8 | NC_004688.1 | + | 8852 | 0.66 | 0.831005 |
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Target: 5'- -aCUGGACGUCgGGguCGcGUUUGUcgaggcgGGCCa -3' miRNA: 3'- aaGACCUGCAGgUC--GC-CGAACA-------UCGG- -5' |
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| 20752 | 5' | -55.8 | NC_004688.1 | + | 20555 | 0.67 | 0.795914 |
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Target: 5'- ---aGGGCGUcgaCCAGCGGCgUGcccuccagGGCCu -3' miRNA: 3'- aagaCCUGCA---GGUCGCCGaACa-------UCGG- -5' |
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| 20752 | 5' | -55.8 | NC_004688.1 | + | 26293 | 0.67 | 0.786507 |
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Target: 5'- -cCUGGGgcUG-CCAGCcuuccucgccaGGCUUGUAGUCg -3' miRNA: 3'- aaGACCU--GCaGGUCG-----------CCGAACAUCGG- -5' |
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| 20752 | 5' | -55.8 | NC_004688.1 | + | 20599 | 0.67 | 0.767257 |
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Target: 5'- -cCUGGccgACGUCagcgaggcugugCAGCGGCUUGUcguAGCg -3' miRNA: 3'- aaGACC---UGCAG------------GUCGCCGAACA---UCGg -5' |
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| 20752 | 5' | -55.8 | NC_004688.1 | + | 41171 | 0.68 | 0.716033 |
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Target: 5'- gUUgUGGACGcCCGGCaGCUUGUcgaggacGGUCu -3' miRNA: 3'- -AAgACCUGCaGGUCGcCGAACA-------UCGG- -5' |
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| 20752 | 5' | -55.8 | NC_004688.1 | + | 91030 | 0.69 | 0.669135 |
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Target: 5'- gUCUGGACGUcgCCccacgccaccacgacAGUGGCU--UGGCCg -3' miRNA: 3'- aAGACCUGCA--GG---------------UCGCCGAacAUCGG- -5' |
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| 20752 | 5' | -55.8 | NC_004688.1 | + | 58757 | 0.7 | 0.622639 |
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Target: 5'- -cCUGGcacauCGUCCGaCGGUggGUGGCCa -3' miRNA: 3'- aaGACCu----GCAGGUcGCCGaaCAUCGG- -5' |
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| 20752 | 5' | -55.8 | NC_004688.1 | + | 82548 | 0.72 | 0.487561 |
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Target: 5'- -gCUGGACGaguuuccacgcuaUCCAGCGGCggcu-GCCg -3' miRNA: 3'- aaGACCUGC-------------AGGUCGCCGaacauCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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