Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20753 | 5' | -51.8 | NC_004688.1 | + | 26768 | 0.69 | 0.877724 |
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Target: 5'- --cGGgGCUGGC-CCGCGGCGU-AGCu -3' miRNA: 3'- uauCUgUGGCUGaGGCGUCGUAaUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 14729 | 0.69 | 0.86204 |
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Target: 5'- --cGACACCcaagaaGGCUCCGCGGCc--GGUg -3' miRNA: 3'- uauCUGUGG------CUGAGGCGUCGuaaUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 93226 | 0.69 | 0.853842 |
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Target: 5'- cGUGGGCACCGAcCUCgGCgaagGGCuc-GGCg -3' miRNA: 3'- -UAUCUGUGGCU-GAGgCG----UCGuaaUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 4096 | 0.69 | 0.851338 |
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Target: 5'- -cAGAUcguCCGAgaggugccccacaaCUCCGCGGCGUaGGCg -3' miRNA: 3'- uaUCUGu--GGCU--------------GAGGCGUCGUAaUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 35354 | 0.72 | 0.719486 |
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Target: 5'- ----cCACCGGCUCCGgCGGCAccGGCc -3' miRNA: 3'- uaucuGUGGCUGAGGC-GUCGUaaUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 65382 | 0.74 | 0.611598 |
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Target: 5'- --cGAUGCCGGCUUCGguGCcgUGGUc -3' miRNA: 3'- uauCUGUGGCUGAGGCguCGuaAUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 39836 | 0.77 | 0.474745 |
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Target: 5'- uGUAGAUuuuGCCGugUCCGguGCcgUGGUu -3' miRNA: 3'- -UAUCUG---UGGCugAGGCguCGuaAUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 44344 | 0.77 | 0.429921 |
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Target: 5'- --cGGCACCGACcggggCCGCAGCGUagaggucgccggcguUGGCg -3' miRNA: 3'- uauCUGUGGCUGa----GGCGUCGUA---------------AUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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