miRNA: 3'- aACGu-----UCgCGCGACGaUCG----UACCGUa -5'
miRNA: 3'- aaCGUUCGCgc-------GACGAUCGU-ACCGUa -5'
miRNA: 3'- -AACGUUCGCGCGACGAUcGUa-CCgUa -5'
miRNA: 3'- aACGUUCGCG----CGACGAUCGUaCCGUa -5'
miRNA: 3'- aACGUUCGCGCGacgAUCGUACCGUa -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20759 | 5' | -56.7 | NC_004688.1 | + | 10930 | 0.67 | 0.6787 |
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Target: 5'- gUGCuucuccAGgGCGCUGCcGGCgcugGUGGCAg -3' miRNA: 3'- aACGu-----UCgCGCGACGaUCG----UACCGUa -5' |
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| 20759 | 5' | -56.7 | NC_004688.1 | + | 100615 | 0.67 | 0.646213 |
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Target: 5'- -gGCAGGCGauucugcuaCUGCUGGCGcUGGCGc -3' miRNA: 3'- aaCGUUCGCgc-------GACGAUCGU-ACCGUa -5' |
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| 20759 | 5' | -56.7 | NC_004688.1 | + | 90819 | 0.7 | 0.447142 |
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Target: 5'- uUUGCGGGCGCGCUGCUGcCAccGGgAg -3' miRNA: 3'- -AACGUUCGCGCGACGAUcGUa-CCgUa -5' |
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| 20759 | 5' | -56.7 | NC_004688.1 | + | 83813 | 0.74 | 0.28683 |
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Target: 5'- gUGCAGGCGUaguuGCUGUUGGCG-GGCGg -3' miRNA: 3'- aACGUUCGCG----CGACGAUCGUaCCGUa -5' |
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| 20759 | 5' | -56.7 | NC_004688.1 | + | 110700 | 0.78 | 0.153228 |
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Target: 5'- cUGCGGGCGCGCg--UGGCAUGGCGa -3' miRNA: 3'- aACGUUCGCGCGacgAUCGUACCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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