Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2076 | 3' | -56.6 | NC_001348.1 | + | 120203 | 0.66 | 0.86962 |
Target: 5'- uCAgGCGGCACGAaGCCCgCGauaACCc -3' miRNA: 3'- -GUgUGCUGUGCUaUGGGgGCgcgUGG- -5' |
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2076 | 3' | -56.6 | NC_001348.1 | + | 109589 | 0.66 | 0.86962 |
Target: 5'- uCAgGCGGCACGAaGCCCgCGauaACCc -3' miRNA: 3'- -GUgUGCUGUGCUaUGGGgGCgcgUGG- -5' |
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2076 | 3' | -56.6 | NC_001348.1 | + | 59838 | 0.66 | 0.876882 |
Target: 5'- aCACACG-C-CGGUACaauuaCCCCGCGacgguaCACCc -3' miRNA: 3'- -GUGUGCuGuGCUAUG-----GGGGCGC------GUGG- -5' |
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2076 | 3' | -56.6 | NC_001348.1 | + | 105901 | 0.66 | 0.876882 |
Target: 5'- cCGCGCGgguacACACGGUGuaugcgacguuCCCaCCGCgGCACa -3' miRNA: 3'- -GUGUGC-----UGUGCUAU-----------GGG-GGCG-CGUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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