Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20760 | 3' | -52 | NC_004689.1 | + | 11350 | 0.66 | 0.924754 |
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Target: 5'- uUGCCGACAU--AC-CCGUCGUa---- -3' miRNA: 3'- -ACGGCUGUGuaUGuGGCAGCAgcaau -5' |
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| 20760 | 3' | -52 | NC_004689.1 | + | 28014 | 0.66 | 0.922983 |
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Target: 5'- uUGCCGACGCAcAUcccggcgaagcaugGCCGUCuGUCa--- -3' miRNA: 3'- -ACGGCUGUGUaUG--------------UGGCAG-CAGcaau -5' |
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| 20760 | 3' | -52 | NC_004689.1 | + | 51712 | 0.66 | 0.912471 |
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Target: 5'- aGCCGcACuCAUGagGCCGUCGUCu--- -3' miRNA: 3'- aCGGC-UGuGUAUg-UGGCAGCAGcaau -5' |
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| 20760 | 3' | -52 | NC_004689.1 | + | 53274 | 0.68 | 0.852556 |
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Target: 5'- cGCCG-Cugu--CGCCGUCGUCGUc- -3' miRNA: 3'- aCGGCuGuguauGUGGCAGCAGCAau -5' |
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| 20760 | 3' | -52 | NC_004689.1 | + | 26526 | 0.7 | 0.725876 |
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Target: 5'- aUGCacgCGACGagcgaaguCGUGCGCCGUUGUCGUc- -3' miRNA: 3'- -ACG---GCUGU--------GUAUGUGGCAGCAGCAau -5' |
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| 20760 | 3' | -52 | NC_004689.1 | + | 1535 | 0.7 | 0.715148 |
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Target: 5'- cGCCuuguGCGCGUGCugCGaaUCGUCGUg- -3' miRNA: 3'- aCGGc---UGUGUAUGugGC--AGCAGCAau -5' |
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| 20760 | 3' | -52 | NC_004689.1 | + | 28853 | 0.71 | 0.693453 |
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Target: 5'- cGCCGugGCAgcCACCGUUGgCGg-- -3' miRNA: 3'- aCGGCugUGUauGUGGCAGCaGCaau -5' |
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| 20760 | 3' | -52 | NC_004689.1 | + | 61532 | 0.73 | 0.572148 |
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Target: 5'- aUGCCacgaauuuuCGCGUACACggCGUCGUCGUUAc -3' miRNA: 3'- -ACGGcu-------GUGUAUGUG--GCAGCAGCAAU- -5' |
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| 20760 | 3' | -52 | NC_004689.1 | + | 2307 | 1.07 | 0.003864 |
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Target: 5'- gUGCCGACACAUACACCGUCGUCGUUAu -3' miRNA: 3'- -ACGGCUGUGUAUGUGGCAGCAGCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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