Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20761 | 5' | -48.6 | NC_004689.1 | + | 12771 | 0.67 | 0.982749 |
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Target: 5'- cGGGAugccUCGGAACA----CGUCUCCc -3' miRNA: 3'- aCUCUu---AGCCUUGUcuuaGCAGAGGc -5' |
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| 20761 | 5' | -48.6 | NC_004689.1 | + | 65556 | 0.67 | 0.978051 |
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Target: 5'- gGAGAacAUCGGcguguagauuucGACGGcggUGUCUCCGg -3' miRNA: 3'- aCUCU--UAGCC------------UUGUCuuaGCAGAGGC- -5' |
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| 20761 | 5' | -48.6 | NC_004689.1 | + | 3917 | 0.67 | 0.972469 |
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Target: 5'- gGAGGguAUCGGGGCAGAGUC--CUCg- -3' miRNA: 3'- aCUCU--UAGCCUUGUCUUAGcaGAGgc -5' |
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| 20761 | 5' | -48.6 | NC_004689.1 | + | 262 | 0.68 | 0.969323 |
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Target: 5'- --cGGAUCGGAGCGGAAUUcugCUCgGc -3' miRNA: 3'- acuCUUAGCCUUGUCUUAGca-GAGgC- -5' |
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| 20761 | 5' | -48.6 | NC_004689.1 | + | 27656 | 0.68 | 0.958364 |
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Target: 5'- gUGAG-GUCGc-GCAGGG-CGUCUCCGa -3' miRNA: 3'- -ACUCuUAGCcuUGUCUUaGCAGAGGC- -5' |
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| 20761 | 5' | -48.6 | NC_004689.1 | + | 31950 | 0.7 | 0.925561 |
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Target: 5'- uUGAGAAUCGGAaccgcaccgcgcauuGCGGAuUCGaUgUCCu -3' miRNA: 3'- -ACUCUUAGCCU---------------UGUCUuAGC-AgAGGc -5' |
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| 20761 | 5' | -48.6 | NC_004689.1 | + | 17168 | 0.7 | 0.903791 |
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Target: 5'- -cAGGAUCGGcgGGCAgGGAUCGcUCUCCa -3' miRNA: 3'- acUCUUAGCC--UUGU-CUUAGC-AGAGGc -5' |
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| 20761 | 5' | -48.6 | NC_004689.1 | + | 58888 | 0.71 | 0.889454 |
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Target: 5'- gGGGAugGUCGGGcCGGGAUCGUCgUgCGg -3' miRNA: 3'- aCUCU--UAGCCUuGUCUUAGCAG-AgGC- -5' |
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| 20761 | 5' | -48.6 | NC_004689.1 | + | 4664 | 1.1 | 0.006779 |
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Target: 5'- aUGAGAAUCGGAACAGAAUCGUCUCCGc -3' miRNA: 3'- -ACUCUUAGCCUUGUCUUAGCAGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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