Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20762 | 3' | -54.8 | NC_004689.1 | + | 12710 | 0.66 | 0.781725 |
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Target: 5'- aCgGUCGCGGGCuGACCGGGcGAc-- -3' miRNA: 3'- aGgUAGCGCCUGuCUGGUUCuCUccg -5' |
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| 20762 | 3' | -54.8 | NC_004689.1 | + | 54743 | 0.67 | 0.771875 |
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Target: 5'- aCCAga-CGGGCcauuuGACCGGG-GAGGCa -3' miRNA: 3'- aGGUagcGCCUGu----CUGGUUCuCUCCG- -5' |
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| 20762 | 3' | -54.8 | NC_004689.1 | + | 34512 | 0.69 | 0.613434 |
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Target: 5'- gCCGUCGCGGAUc-AUC--GGGAGGCa -3' miRNA: 3'- aGGUAGCGCCUGucUGGuuCUCUCCG- -5' |
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| 20762 | 3' | -54.8 | NC_004689.1 | + | 11921 | 0.7 | 0.590776 |
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Target: 5'- gCCGUCGCGcGagaaagcGCGGAUCAugcGGAGGCa -3' miRNA: 3'- aGGUAGCGC-C-------UGUCUGGUuc-UCUCCG- -5' |
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| 20762 | 3' | -54.8 | NC_004689.1 | + | 12203 | 0.72 | 0.46715 |
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Target: 5'- -aCGUCGaUGGGCAGACCGGcaccGcGAGGCa -3' miRNA: 3'- agGUAGC-GCCUGUCUGGUU----CuCUCCG- -5' |
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| 20762 | 3' | -54.8 | NC_004689.1 | + | 16666 | 0.76 | 0.273803 |
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Target: 5'- uUCCAcgcUUGCGGACcucGACCucgcgguAGGGAGGCa -3' miRNA: 3'- -AGGU---AGCGCCUGu--CUGGu------UCUCUCCG- -5' |
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| 20762 | 3' | -54.8 | NC_004689.1 | + | 6058 | 1.13 | 0.000838 |
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Target: 5'- gUCCAUCGCGGACAGACCAAGAGAGGCg -3' miRNA: 3'- -AGGUAGCGCCUGUCUGGUUCUCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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