Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20762 | 5' | -56.1 | NC_004689.1 | + | 67712 | 0.66 | 0.703822 |
Target: 5'- -cGCC-CAGCCGuaGC-GCgAUGUUGGCGc -3' miRNA: 3'- gaCGGaGUCGGU--UGuCG-UACAGCCGU- -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 28703 | 0.66 | 0.689831 |
Target: 5'- -gGUCUCGGCaguauccaccguauCGGCGGCuugGUUGGCAa -3' miRNA: 3'- gaCGGAGUCG--------------GUUGUCGua-CAGCCGU- -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 41465 | 0.66 | 0.682258 |
Target: 5'- -gGUCUCGGaCCAcgaccGCAGCGUcUCGGUg -3' miRNA: 3'- gaCGGAGUC-GGU-----UGUCGUAcAGCCGu -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 29082 | 0.66 | 0.6714 |
Target: 5'- -gGCacacgaUCAGCCGugAGCAUGUUGuaGg -3' miRNA: 3'- gaCGg-----AGUCGGUugUCGUACAGCcgU- -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 35000 | 0.66 | 0.6714 |
Target: 5'- -cGCCUUGGCCGuu-GCccuUGUCGGUg -3' miRNA: 3'- gaCGGAGUCGGUuguCGu--ACAGCCGu -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 14297 | 0.68 | 0.59494 |
Target: 5'- -gGCCUCGuCC--CAGUGUGUCGGUAa -3' miRNA: 3'- gaCGGAGUcGGuuGUCGUACAGCCGU- -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 13966 | 0.69 | 0.488905 |
Target: 5'- -cGCCUCGGCCuugacCGGCuccgcGUCGGUg -3' miRNA: 3'- gaCGGAGUCGGuu---GUCGua---CAGCCGu -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 64597 | 0.7 | 0.467704 |
Target: 5'- -aGCUUgaagauuccccaaCAGCCAgggcACAGCAUGaUCGGCGg -3' miRNA: 3'- gaCGGA-------------GUCGGU----UGUCGUAC-AGCCGU- -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 48893 | 0.71 | 0.410842 |
Target: 5'- -gGCCUCGGCCugugccucGGCGGCAcGUgCGGCc -3' miRNA: 3'- gaCGGAGUCGG--------UUGUCGUaCA-GCCGu -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 66690 | 0.71 | 0.392561 |
Target: 5'- -cGCCUgAuCCAACAGCA-GUUGGCGu -3' miRNA: 3'- gaCGGAgUcGGUUGUCGUaCAGCCGU- -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 59966 | 0.74 | 0.280148 |
Target: 5'- uCUGUgUCGGCCAACAGUucGUCGGgAu -3' miRNA: 3'- -GACGgAGUCGGUUGUCGuaCAGCCgU- -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 27769 | 0.74 | 0.253109 |
Target: 5'- -cGCCcCAGUCugcgccaccCAGCAUGUCGGCAa -3' miRNA: 3'- gaCGGaGUCGGuu-------GUCGUACAGCCGU- -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 12561 | 0.75 | 0.246694 |
Target: 5'- gUG-CUCGGCCAGCAGCAUGUaaagGGUg -3' miRNA: 3'- gACgGAGUCGGUUGUCGUACAg---CCGu -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 35105 | 0.77 | 0.165832 |
Target: 5'- uCUGauccaCUguGCCAGCGGCgAUGUCGGCGu -3' miRNA: 3'- -GACg----GAguCGGUUGUCG-UACAGCCGU- -5' |
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20762 | 5' | -56.1 | NC_004689.1 | + | 6024 | 1.08 | 0.000994 |
Target: 5'- uCUGCCUCAGCCAACAGCAUGUCGGCAa -3' miRNA: 3'- -GACGGAGUCGGUUGUCGUACAGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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