Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20763 | 5' | -52.8 | NC_004689.1 | + | 22876 | 0.66 | 0.894386 |
Target: 5'- --uCUUCGGCGGcuGCGAUCUGuuguuccuucGACGc -3' miRNA: 3'- gguGAGGCCGUCuuCGCUAGAC----------UUGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 19814 | 0.66 | 0.892262 |
Target: 5'- gCCGCUUCGGUcugcgaacauggucAGAAGCGccCaGAGCGc -3' miRNA: 3'- -GGUGAGGCCG--------------UCUUCGCuaGaCUUGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 25552 | 0.67 | 0.87211 |
Target: 5'- aCACUUCGGCGGGaucaacAGCGAUggggUUGAAg- -3' miRNA: 3'- gGUGAGGCCGUCU------UCGCUA----GACUUgc -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 4020 | 0.67 | 0.864188 |
Target: 5'- cCCAC-CCGuGUGGggGCGA---GAACGg -3' miRNA: 3'- -GGUGaGGC-CGUCuuCGCUagaCUUGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 11416 | 0.67 | 0.864188 |
Target: 5'- cCCA--CCGGCAGAAcGCGGUuggcCUGAgugGCGa -3' miRNA: 3'- -GGUgaGGCCGUCUU-CGCUA----GACU---UGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 53826 | 0.67 | 0.864188 |
Target: 5'- uCCAUgagCGcGCAGAAGCGGa-UGAACGg -3' miRNA: 3'- -GGUGag-GC-CGUCUUCGCUagACUUGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 62847 | 0.67 | 0.864188 |
Target: 5'- cUCAUUCCGGUAGggGUuGUgCaGGGCGa -3' miRNA: 3'- -GGUGAGGCCGUCuuCGcUA-GaCUUGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 9924 | 0.67 | 0.856028 |
Target: 5'- -gGCUCCGGCuuuuguGGAGUGAugaaaUCgcgUGAGCGa -3' miRNA: 3'- ggUGAGGCCGu-----CUUCGCU-----AG---ACUUGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 29226 | 0.67 | 0.856028 |
Target: 5'- cCCACUCCGGUAcGAAucGCaGAUggccgGAACGg -3' miRNA: 3'- -GGUGAGGCCGU-CUU--CG-CUAga---CUUGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 10645 | 0.69 | 0.777266 |
Target: 5'- uCCACaCUGGCAGccccgccaggcuguuGGGCGGUCUgcgGAGCGc -3' miRNA: 3'- -GGUGaGGCCGUC---------------UUCGCUAGA---CUUGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 17112 | 0.69 | 0.773286 |
Target: 5'- aCCACaCCGGCccugcgguGAGGCcGGUCUGAgugACGu -3' miRNA: 3'- -GGUGaGGCCGu-------CUUCG-CUAGACU---UGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 42160 | 0.69 | 0.763237 |
Target: 5'- cCUGCgCUGGCGGGgauGCGAUCaGAGCGc -3' miRNA: 3'- -GGUGaGGCCGUCUu--CGCUAGaCUUGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 16909 | 0.7 | 0.700556 |
Target: 5'- uUCGCUCCGGCGucAGCGAaaUGGcCGa -3' miRNA: 3'- -GGUGAGGCCGUcuUCGCUagACUuGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 33680 | 0.71 | 0.668182 |
Target: 5'- uCgGCUCCGGCAGcGGCaucgGggUUGAGCGu -3' miRNA: 3'- -GgUGAGGCCGUCuUCG----CuaGACUUGC- -5' |
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20763 | 5' | -52.8 | NC_004689.1 | + | 6821 | 1.11 | 0.002048 |
Target: 5'- cCCACUCCGGCAGAAGCGAUCUGAACGu -3' miRNA: 3'- -GGUGAGGCCGUCUUCGCUAGACUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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