miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20764 3' -59 NC_004689.1 + 11083 0.66 0.611381
Target:  5'- -aGCCgcUGGCGGGGUCUGCuGCUG-CGg -3'
miRNA:   3'- ugUGG--GCCGCUCCAGGUGcUGACuGC- -5'
20764 3' -59 NC_004689.1 + 66195 0.67 0.569816
Target:  5'- gUACCCGGUagcuGGGUUCGCGuggcucACUGugGg -3'
miRNA:   3'- uGUGGGCCGc---UCCAGGUGC------UGACugC- -5'
20764 3' -59 NC_004689.1 + 35600 0.68 0.459478
Target:  5'- aGCACCCGGCccaGGG-CUGCGaaggaucagcaccGCUGGCGg -3'
miRNA:   3'- -UGUGGGCCGc--UCCaGGUGC-------------UGACUGC- -5'
20764 3' -59 NC_004689.1 + 56297 0.73 0.236988
Target:  5'- gUACCCGGUGuuGUUCGCGACacUGGCGa -3'
miRNA:   3'- uGUGGGCCGCucCAGGUGCUG--ACUGC- -5'
20764 3' -59 NC_004689.1 + 8146 1.08 0.000866
Target:  5'- gACACCCGGCGAGGUCCACGACUGACGu -3'
miRNA:   3'- -UGUGGGCCGCUCCAGGUGCUGACUGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.