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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20764 | 5' | -55.6 | NC_004689.1 | + | 37608 | 0.66 | 0.775092 |
Target: 5'- gUGCGUucaACGGUcGCUGugUaauuGUGGGUGu -3' miRNA: 3'- -ACGCA---UGUCAaCGGCugGg---UACCCAC- -5' |
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20764 | 5' | -55.6 | NC_004689.1 | + | 31640 | 0.66 | 0.755012 |
Target: 5'- gGCGUGcCAGgcaaUGCCGACaCCAcagacGGUGa -3' miRNA: 3'- aCGCAU-GUCa---ACGGCUG-GGUac---CCAC- -5' |
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20764 | 5' | -55.6 | NC_004689.1 | + | 8112 | 1.08 | 0.001451 |
Target: 5'- aUGCGUACAGUUGCCGACCCAUGGGUGc -3' miRNA: 3'- -ACGCAUGUCAACGGCUGGGUACCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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