Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20765 | 3' | -56.1 | NC_004689.1 | + | 49546 | 0.66 | 0.752885 |
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Target: 5'- -aUUCUUGGCGUcCuCGGgacGUGGUugCu -3' miRNA: 3'- ggGAGAACCGCAuG-GCCa--CGCCAugG- -5' |
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| 20765 | 3' | -56.1 | NC_004689.1 | + | 12729 | 0.67 | 0.722559 |
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Target: 5'- aCCCUC--GGCGUuggggagaaCGGUcGCGGgcugACCg -3' miRNA: 3'- -GGGAGaaCCGCAug-------GCCA-CGCCa---UGG- -5' |
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| 20765 | 3' | -56.1 | NC_004689.1 | + | 52292 | 0.67 | 0.700868 |
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Target: 5'- aCCgUCUUgGGCGUgagcgagACC-GUGCGGU-CCu -3' miRNA: 3'- -GGgAGAA-CCGCA-------UGGcCACGCCAuGG- -5' |
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| 20765 | 3' | -56.1 | NC_004689.1 | + | 59258 | 0.68 | 0.681005 |
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Target: 5'- cCCCUCaUGGac-GCCGGUcccugcucGCGGcACCa -3' miRNA: 3'- -GGGAGaACCgcaUGGCCA--------CGCCaUGG- -5' |
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| 20765 | 3' | -56.1 | NC_004689.1 | + | 58756 | 0.69 | 0.585879 |
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Target: 5'- aCCCUUUUGGCGUgagccgagucgaGCCGGaacaGCGc-ACCa -3' miRNA: 3'- -GGGAGAACCGCA------------UGGCCa---CGCcaUGG- -5' |
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| 20765 | 3' | -56.1 | NC_004689.1 | + | 41964 | 0.73 | 0.365195 |
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Target: 5'- gCCUUCUUGGCGcgggacuugGCCGGUGCcuucguGGUcggagcACCg -3' miRNA: 3'- -GGGAGAACCGCa--------UGGCCACG------CCA------UGG- -5' |
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| 20765 | 3' | -56.1 | NC_004689.1 | + | 11967 | 1.14 | 0.000602 |
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Target: 5'- cCCCUCUUGGCGUACCGGUGCGGUACCg -3' miRNA: 3'- -GGGAGAACCGCAUGGCCACGCCAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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