Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20766 | 3' | -62.1 | NC_004689.1 | + | 9508 | 0.66 | 0.428994 |
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Target: 5'- gAGGuCUGGuCAUCGUGGGGCAaugGCCGGg -3' miRNA: 3'- gUCU-GGCC-GUGGCGCUCCGUg--CGGUU- -5' |
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| 20766 | 3' | -62.1 | NC_004689.1 | + | 11953 | 0.66 | 0.411004 |
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Target: 5'- cCGGugCGGUACCGCcgccgaGAGaacccCACGCCGu -3' miRNA: 3'- -GUCugGCCGUGGCG------CUCc----GUGCGGUu -5' |
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| 20766 | 3' | -62.1 | NC_004689.1 | + | 11123 | 0.66 | 0.411004 |
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Target: 5'- -uGGCCGGgggaGCCG-GAGGCuuGCCGGa -3' miRNA: 3'- guCUGGCCg---UGGCgCUCCGugCGGUU- -5' |
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| 20766 | 3' | -62.1 | NC_004689.1 | + | 42169 | 0.66 | 0.38833 |
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Target: 5'- cCGGAUCGGC-CUGCGcuGGCggggaugcgaucagaGCGCCGAc -3' miRNA: 3'- -GUCUGGCCGuGGCGCu-CCG---------------UGCGGUU- -5' |
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| 20766 | 3' | -62.1 | NC_004689.1 | + | 35578 | 0.68 | 0.320904 |
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Target: 5'- aGGAUCaGCACCGCuggcGGGCAaGCCAAu -3' miRNA: 3'- gUCUGGcCGUGGCGc---UCCGUgCGGUU- -5' |
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| 20766 | 3' | -62.1 | NC_004689.1 | + | 27172 | 0.68 | 0.299042 |
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Target: 5'- -uGACCGGCACCGUGAuucGGCcuuguuCGUCu- -3' miRNA: 3'- guCUGGCCGUGGCGCU---CCGu-----GCGGuu -5' |
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| 20766 | 3' | -62.1 | NC_004689.1 | + | 19155 | 0.73 | 0.133117 |
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Target: 5'- uCAGACCgGGCAgUGCGAGGaaccCGCCAGc -3' miRNA: 3'- -GUCUGG-CCGUgGCGCUCCgu--GCGGUU- -5' |
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| 20766 | 3' | -62.1 | NC_004689.1 | + | 30171 | 0.79 | 0.054291 |
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Target: 5'- cCAGACCGGCACCGCuGGcGUugGCUAu -3' miRNA: 3'- -GUCUGGCCGUGGCGcUC-CGugCGGUu -5' |
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| 20766 | 3' | -62.1 | NC_004689.1 | + | 12193 | 1.05 | 0.000508 |
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Target: 5'- gCAGACCGGCACCGCGAGGCACGCCAAg -3' miRNA: 3'- -GUCUGGCCGUGGCGCUCCGUGCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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