miRNA display CGI


Results 1 - 3 of 3 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20767 3' -53.9 NC_004689.1 + 8121 0.67 0.772883
Target:  5'- --cGUUGCGGGAU-GCGUacAGUUGCc- -3'
miRNA:   3'- ugaCGACGCCCUAgUGCA--UCAACGac -5'
20767 3' -53.9 NC_004689.1 + 11067 0.69 0.655303
Target:  5'- uGCUGCUGCGGGuuguuCGgaucgGGcUGCUGc -3'
miRNA:   3'- -UGACGACGCCCuagu-GCa----UCaACGAC- -5'
20767 3' -53.9 NC_004689.1 + 13066 1.08 0.001993
Target:  5'- aACUGCUGCGGGAUCACGUAGUUGCUGu -3'
miRNA:   3'- -UGACGACGCCCUAGUGCAUCAACGAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.