Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20767 | 5' | -54.2 | NC_004689.1 | + | 21696 | 0.66 | 0.807337 |
Target: 5'- cGGUGCCCcaGGCUGCAcaggGAcCGUGGCAg -3' miRNA: 3'- -UCGUGGG--UUGACGUa---CUaGCGUCGUg -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 18249 | 0.66 | 0.787783 |
Target: 5'- uAGCGCCCGAuCUGCc--AUCuuGGCACc -3' miRNA: 3'- -UCGUGGGUU-GACGuacUAGcgUCGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 7212 | 0.66 | 0.777752 |
Target: 5'- gAGCAgaagCCAACUGCGccuuacgcaaUGcugCGCGGCGCu -3' miRNA: 3'- -UCGUg---GGUUGACGU----------ACua-GCGUCGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 18670 | 0.66 | 0.777752 |
Target: 5'- uGCGCCgguugccgguCAACUGguUGAcCGCuGCGCc -3' miRNA: 3'- uCGUGG----------GUUGACguACUaGCGuCGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 275 | 0.66 | 0.75724 |
Target: 5'- cGGCACCau-UUGUcgGAUCGgAGCGg -3' miRNA: 3'- -UCGUGGguuGACGuaCUAGCgUCGUg -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 35046 | 0.67 | 0.746782 |
Target: 5'- uAGCucACCCAACcgccUGCAaccaucUGGUCGC-GCGCc -3' miRNA: 3'- -UCG--UGGGUUG----ACGU------ACUAGCGuCGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 35601 | 0.68 | 0.669803 |
Target: 5'- aAGCACCCGgcccaggGCUGCGaagGAUCaGCAcCGCu -3' miRNA: 3'- -UCGUGGGU-------UGACGUa--CUAG-CGUcGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 23149 | 0.69 | 0.593166 |
Target: 5'- aAGcCAUUCGGCUGCGU--UCGCAGUGCu -3' miRNA: 3'- -UC-GUGGGUUGACGUAcuAGCGUCGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 39197 | 0.71 | 0.484495 |
Target: 5'- cGGUugCCGcugcACUGCAUcGGUCGCAucgauccGCACg -3' miRNA: 3'- -UCGugGGU----UGACGUA-CUAGCGU-------CGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 18940 | 0.71 | 0.465061 |
Target: 5'- uGCugCCAGCcGCGUGccauaagCGCAGUACc -3' miRNA: 3'- uCGugGGUUGaCGUACua-----GCGUCGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 64526 | 0.73 | 0.361443 |
Target: 5'- uGCACUCAugU-CAUGG-CGCAGCACu -3' miRNA: 3'- uCGUGGGUugAcGUACUaGCGUCGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 7877 | 0.74 | 0.327956 |
Target: 5'- cGCGCagagaCCAACUGCGccuuUCGCAGCGCa -3' miRNA: 3'- uCGUG-----GGUUGACGUacu-AGCGUCGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 59094 | 0.75 | 0.289404 |
Target: 5'- cGCACCUGAUcGCAgUGAcCGCAGCACu -3' miRNA: 3'- uCGUGGGUUGaCGU-ACUaGCGUCGUG- -5' |
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20767 | 5' | -54.2 | NC_004689.1 | + | 13032 | 1.09 | 0.001347 |
Target: 5'- gAGCACCCAACUGCAUGAUCGCAGCACc -3' miRNA: 3'- -UCGUGGGUUGACGUACUAGCGUCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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