Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20768 | 3' | -55.6 | NC_004689.1 | + | 34519 | 0.66 | 0.797841 |
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Target: 5'- cACG-CCUgCCGUCGCGGaucauCGGGa--GGCa -3' miRNA: 3'- -UGCaGGA-GGCAGCGUC-----GCCUaaaCCG- -5' |
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| 20768 | 3' | -55.6 | NC_004689.1 | + | 44900 | 0.68 | 0.664941 |
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Target: 5'- uUGUCUgugUCGUCGCuGCGGAgcUUGcGCg -3' miRNA: 3'- uGCAGGa--GGCAGCGuCGCCUa-AAC-CG- -5' |
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| 20768 | 3' | -55.6 | NC_004689.1 | + | 28862 | 0.69 | 0.62199 |
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Target: 5'- cACGUUCgucgCCGUgGCAGCcaccgUUGGCg -3' miRNA: 3'- -UGCAGGa---GGCAgCGUCGccua-AACCG- -5' |
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| 20768 | 3' | -55.6 | NC_004689.1 | + | 17032 | 0.72 | 0.450609 |
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Target: 5'- cCGUCCU-CGUCGuCAGCGGAaUagaugccguacucggUGGCa -3' miRNA: 3'- uGCAGGAgGCAGC-GUCGCCUaA---------------ACCG- -5' |
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| 20768 | 3' | -55.6 | NC_004689.1 | + | 54319 | 0.73 | 0.365612 |
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Target: 5'- -aGUCUUCUGcCGCAGCaGAUUcGGCg -3' miRNA: 3'- ugCAGGAGGCaGCGUCGcCUAAaCCG- -5' |
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| 20768 | 3' | -55.6 | NC_004689.1 | + | 14173 | 1.1 | 0.001106 |
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Target: 5'- aACGUCCUCCGUCGCAGCGGAUUUGGCc -3' miRNA: 3'- -UGCAGGAGGCAGCGUCGCCUAAACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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