Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20768 | 5' | -54.1 | NC_004689.1 | + | 25901 | 0.66 | 0.843277 |
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Target: 5'- -gGCCAACuCCGCUu-GAUcaGGUUUUCGc -3' miRNA: 3'- agCGGUUG-GGCGGucCUA--CCAAAAGC- -5' |
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| 20768 | 5' | -54.1 | NC_004689.1 | + | 70724 | 0.66 | 0.834574 |
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Target: 5'- cCGCUugcaAACCCGCCAGGGgacaGUccUUUCc -3' miRNA: 3'- aGCGG----UUGGGCGGUCCUac--CA--AAAGc -5' |
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| 20768 | 5' | -54.1 | NC_004689.1 | + | 15964 | 0.67 | 0.768356 |
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Target: 5'- uUCGCCuGCaCGCCGGGAUGa---UCGa -3' miRNA: 3'- -AGCGGuUGgGCGGUCCUACcaaaAGC- -5' |
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| 20768 | 5' | -54.1 | NC_004689.1 | + | 50959 | 0.68 | 0.748042 |
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Target: 5'- -gGCC--UCUGCCAGuuUGGUUUUCGa -3' miRNA: 3'- agCGGuuGGGCGGUCcuACCAAAAGC- -5' |
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| 20768 | 5' | -54.1 | NC_004689.1 | + | 59958 | 0.68 | 0.716736 |
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Target: 5'- -gGCCAACaguuCGUCGGGAUGGcacgUCGc -3' miRNA: 3'- agCGGUUGg---GCGGUCCUACCaaa-AGC- -5' |
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| 20768 | 5' | -54.1 | NC_004689.1 | + | 41558 | 0.68 | 0.716736 |
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Target: 5'- cUCGCCAGCCuCGgaCAGGAUGacc-UCGg -3' miRNA: 3'- -AGCGGUUGG-GCg-GUCCUACcaaaAGC- -5' |
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| 20768 | 5' | -54.1 | NC_004689.1 | + | 54783 | 0.7 | 0.619555 |
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Target: 5'- cCGUaGACCCGCCAGGGcguUGGc--UCGg -3' miRNA: 3'- aGCGgUUGGGCGGUCCU---ACCaaaAGC- -5' |
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| 20768 | 5' | -54.1 | NC_004689.1 | + | 14138 | 1.09 | 0.001874 |
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Target: 5'- cUCGCCAACCCGCCAGGAUGGUUUUCGu -3' miRNA: 3'- -AGCGGUUGGGCGGUCCUACCAAAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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