Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20770 | 5' | -53.2 | NC_004689.1 | + | 21669 | 0.66 | 0.883987 |
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Target: 5'- gGCAgg-CGG-UGCCGgagguCCACCGGGg -3' miRNA: 3'- gUGUgaaGCCaACGGUau---GGUGGCCU- -5' |
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| 20770 | 5' | -53.2 | NC_004689.1 | + | 20948 | 0.66 | 0.8764 |
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Target: 5'- aUAC-CUUCGGcgaGCUuggcACCACCGGGa -3' miRNA: 3'- -GUGuGAAGCCaa-CGGua--UGGUGGCCU- -5' |
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| 20770 | 5' | -53.2 | NC_004689.1 | + | 40670 | 0.66 | 0.868561 |
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Target: 5'- aGCAUUUCGGcgUUGaUCAUcGCCugCGGGa -3' miRNA: 3'- gUGUGAAGCC--AAC-GGUA-UGGugGCCU- -5' |
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| 20770 | 5' | -53.2 | NC_004689.1 | + | 10555 | 0.67 | 0.834835 |
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Target: 5'- uGCugUUCGGgauugGCCGgaggggugGCCguuGCCGGGg -3' miRNA: 3'- gUGugAAGCCaa---CGGUa-------UGG---UGGCCU- -5' |
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| 20770 | 5' | -53.2 | NC_004689.1 | + | 397 | 0.68 | 0.787987 |
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Target: 5'- aCGCACaUUCGGcUGCgGgaauuUCGCCGGAg -3' miRNA: 3'- -GUGUG-AAGCCaACGgUau---GGUGGCCU- -5' |
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| 20770 | 5' | -53.2 | NC_004689.1 | + | 45532 | 0.68 | 0.757858 |
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Target: 5'- uCACgAUUUCGG--GCCgGUGCUGCCGGAg -3' miRNA: 3'- -GUG-UGAAGCCaaCGG-UAUGGUGGCCU- -5' |
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| 20770 | 5' | -53.2 | NC_004689.1 | + | 16590 | 1.09 | 0.002243 |
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Target: 5'- cCACACUUCGGUUGCCAUACCACCGGAu -3' miRNA: 3'- -GUGUGAAGCCAACGGUAUGGUGGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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