Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20771 | 3' | -53.8 | NC_004689.1 | + | 25895 | 0.66 | 0.836565 |
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Target: 5'- cUCCGC--UUGAUCAGGuuuUCGCCgGa-- -3' miRNA: 3'- -AGGCGugAACUAGUCC---AGCGGgUaua -5' |
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| 20771 | 3' | -53.8 | NC_004689.1 | + | 12789 | 0.66 | 0.836565 |
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Target: 5'- cCCGUACUugccgaUGAUCGGGaUGCCUcgGa -3' miRNA: 3'- aGGCGUGA------ACUAGUCCaGCGGGuaUa -5' |
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| 20771 | 3' | -53.8 | NC_004689.1 | + | 41147 | 0.66 | 0.808905 |
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Target: 5'- aCCGCGCagGAUCAGcG-CGCUCGUc- -3' miRNA: 3'- aGGCGUGaaCUAGUC-CaGCGGGUAua -5' |
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| 20771 | 3' | -53.8 | NC_004689.1 | + | 32344 | 0.67 | 0.769453 |
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Target: 5'- gCCGUGCUUGAUUucacugcuGGUCGgCCAg-- -3' miRNA: 3'- aGGCGUGAACUAGu-------CCAGCgGGUaua -5' |
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| 20771 | 3' | -53.8 | NC_004689.1 | + | 51444 | 0.72 | 0.49918 |
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Target: 5'- cCCGCGuCgaacgGAUCGGGUgCGCCCAa-- -3' miRNA: 3'- aGGCGU-Gaa---CUAGUCCA-GCGGGUaua -5' |
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| 20771 | 3' | -53.8 | NC_004689.1 | + | 17480 | 1.06 | 0.00272 |
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Target: 5'- uUCCGCACUUGAUCAGGUCGCCCAUAUc -3' miRNA: 3'- -AGGCGUGAACUAGUCCAGCGGGUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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