Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20772 | 5' | -51.1 | NC_004689.1 | + | 13325 | 0.66 | 0.914775 |
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Target: 5'- cGGUGGCucACCGGCCUGUucguGCGGC-AGAg -3' miRNA: 3'- -CUAUUG--UGGUCGGACG----UGUUGcUCUa -5' |
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| 20772 | 5' | -51.1 | NC_004689.1 | + | 37137 | 0.66 | 0.914775 |
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Target: 5'- --cAGCGCCGGUCUGCgucccGCGugGuGAg -3' miRNA: 3'- cuaUUGUGGUCGGACG-----UGUugCuCUa -5' |
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| 20772 | 5' | -51.1 | NC_004689.1 | + | 33807 | 0.66 | 0.901211 |
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Target: 5'- --cAACugCAacuucGCCUGCGCAACGcuggGGAa -3' miRNA: 3'- cuaUUGugGU-----CGGACGUGUUGC----UCUa -5' |
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| 20772 | 5' | -51.1 | NC_004689.1 | + | 11478 | 0.67 | 0.868209 |
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Target: 5'- --cAACGCCgacgauuccggucuGGCCUGCGCcacCGAGAa -3' miRNA: 3'- cuaUUGUGG--------------UCGGACGUGuu-GCUCUa -5' |
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| 20772 | 5' | -51.1 | NC_004689.1 | + | 42172 | 0.68 | 0.817138 |
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Target: 5'- --gGACcggAUCGGCCUGCGCuGGCGGGGa -3' miRNA: 3'- cuaUUG---UGGUCGGACGUG-UUGCUCUa -5' |
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| 20772 | 5' | -51.1 | NC_004689.1 | + | 45145 | 0.71 | 0.690338 |
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Target: 5'- uGAUGGCAUCgAGCCUG-ACAGCGuGAa -3' miRNA: 3'- -CUAUUGUGG-UCGGACgUGUUGCuCUa -5' |
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| 20772 | 5' | -51.1 | NC_004689.1 | + | 17642 | 1.05 | 0.00506 |
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Target: 5'- gGAUAACACCAGCCUGCACAACGAGAUu -3' miRNA: 3'- -CUAUUGUGGUCGGACGUGUUGCUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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