Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20773 | 5' | -57.2 | NC_004689.1 | + | 60922 | 0.66 | 0.701908 |
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Target: 5'- gUGGcgcguGUCGAUCU--GUCCCcAGCGCCAu -3' miRNA: 3'- -ACC-----UAGCUAGGcgCAGGGcUUGCGGU- -5' |
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| 20773 | 5' | -57.2 | NC_004689.1 | + | 42148 | 0.66 | 0.691483 |
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Target: 5'- gGGAUgCGAUCagaGCG--CCGAcaGCGCCAa -3' miRNA: 3'- aCCUA-GCUAGg--CGCagGGCU--UGCGGU- -5' |
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| 20773 | 5' | -57.2 | NC_004689.1 | + | 52179 | 0.66 | 0.691483 |
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Target: 5'- cGGAUCGggCUGCGugacuUCCUcGACGCgCAu -3' miRNA: 3'- aCCUAGCuaGGCGC-----AGGGcUUGCG-GU- -5' |
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| 20773 | 5' | -57.2 | NC_004689.1 | + | 25643 | 0.67 | 0.64935 |
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Target: 5'- gGGAgc-AUCUGCGUUCCGGuugAUGCCGg -3' miRNA: 3'- aCCUagcUAGGCGCAGGGCU---UGCGGU- -5' |
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| 20773 | 5' | -57.2 | NC_004689.1 | + | 62602 | 0.68 | 0.585879 |
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Target: 5'- ---uUCGAgccaCUGCGUUCCGAcuGCGCCGa -3' miRNA: 3'- accuAGCUa---GGCGCAGGGCU--UGCGGU- -5' |
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| 20773 | 5' | -57.2 | NC_004689.1 | + | 47985 | 0.68 | 0.575379 |
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Target: 5'- cUGGAUCGGUgaGCuUUUCGAAUGCCu -3' miRNA: 3'- -ACCUAGCUAggCGcAGGGCUUGCGGu -5' |
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| 20773 | 5' | -57.2 | NC_004689.1 | + | 1784 | 0.68 | 0.554517 |
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Target: 5'- aUGGGUCGGguugccuugCCGCGUCCggUGAACaGCUu -3' miRNA: 3'- -ACCUAGCUa--------GGCGCAGG--GCUUG-CGGu -5' |
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| 20773 | 5' | -57.2 | NC_004689.1 | + | 58873 | 0.69 | 0.544168 |
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Target: 5'- gGGAUCG-UCgUGCGgaaCGAACGCCAu -3' miRNA: 3'- aCCUAGCuAG-GCGCaggGCUUGCGGU- -5' |
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| 20773 | 5' | -57.2 | NC_004689.1 | + | 25446 | 0.7 | 0.453618 |
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Target: 5'- cGGAUCGAUCUucgGCGggCCUGuaauuccAGCGCCGa -3' miRNA: 3'- aCCUAGCUAGG---CGCa-GGGC-------UUGCGGU- -5' |
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| 20773 | 5' | -57.2 | NC_004689.1 | + | 18416 | 1.08 | 0.001167 |
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Target: 5'- gUGGAUCGAUCCGCGUCCCGAACGCCAc -3' miRNA: 3'- -ACCUAGCUAGGCGCAGGGCUUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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