Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20774 | 3' | -56.1 | NC_004689.1 | + | 4413 | 0.66 | 0.675218 |
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Target: 5'- aGGGGuaucaccauACCCGCCAGCUugg-GCUUuGCg -3' miRNA: 3'- -UCCU---------UGGGCGGUCGGucaaCGAAcUG- -5' |
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| 20774 | 3' | -56.1 | NC_004689.1 | + | 44871 | 0.68 | 0.588008 |
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Target: 5'- cGGAucggGCCUGCCGGUCAcc-GCUUGAg -3' miRNA: 3'- uCCU----UGGGCGGUCGGUcaaCGAACUg -5' |
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| 20774 | 3' | -56.1 | NC_004689.1 | + | 54133 | 0.68 | 0.588008 |
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Target: 5'- cGGAGgCCG-CAGCCAcGUUG-UUGACa -3' miRNA: 3'- uCCUUgGGCgGUCGGU-CAACgAACUG- -5' |
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| 20774 | 3' | -56.1 | NC_004689.1 | + | 20585 | 0.69 | 0.492745 |
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Target: 5'- cGGGGACCUGCuCGGCCu-UUGCaUUGAg -3' miRNA: 3'- -UCCUUGGGCG-GUCGGucAACG-AACUg -5' |
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| 20774 | 3' | -56.1 | NC_004689.1 | + | 57860 | 0.73 | 0.319464 |
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Target: 5'- cGGAACCCGCCaAGCuguguggCAGUUcGgUUGACa -3' miRNA: 3'- uCCUUGGGCGG-UCG-------GUCAA-CgAACUG- -5' |
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| 20774 | 3' | -56.1 | NC_004689.1 | + | 44509 | 0.75 | 0.236834 |
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Target: 5'- uGGGcaGACCUGUgAGCCAGUUcaucaGCUUGGCg -3' miRNA: 3'- -UCC--UUGGGCGgUCGGUCAA-----CGAACUG- -5' |
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| 20774 | 3' | -56.1 | NC_004689.1 | + | 19139 | 1.09 | 0.000985 |
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Target: 5'- gAGGAACCCGCCAGCCAGUUGCUUGACc -3' miRNA: 3'- -UCCUUGGGCGGUCGGUCAACGAACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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