Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20774 | 5' | -49.3 | NC_004689.1 | + | 2845 | 0.66 | 0.979101 |
Target: 5'- uGCcCCGAau-GCGGG-UCGAAGACCu -3' miRNA: 3'- -CGuGGCUcguUGUCCaAGCUUUUGGu -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 51278 | 0.66 | 0.973692 |
Target: 5'- aCACCG-GCuGCuGGUUCGc-GGCCAa -3' miRNA: 3'- cGUGGCuCGuUGuCCAAGCuuUUGGU- -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 3760 | 0.67 | 0.968685 |
Target: 5'- cGCugCGAugguuucccaguccuGCGACGGGUU-GuAAACCAg -3' miRNA: 3'- -CGugGCU---------------CGUUGUCCAAgCuUUUGGU- -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 29665 | 0.67 | 0.959959 |
Target: 5'- uGCACCG-GCAcCAG---CGGGAGCCAg -3' miRNA: 3'- -CGUGGCuCGUuGUCcaaGCUUUUGGU- -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 12140 | 0.67 | 0.955871 |
Target: 5'- uCACCGAucuGCAcgACcGGUUCGAccgGAACCu -3' miRNA: 3'- cGUGGCU---CGU--UGuCCAAGCU---UUUGGu -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 6599 | 0.67 | 0.951509 |
Target: 5'- cCACCcaGAGCA--GGGUgauacCGAAGACCAg -3' miRNA: 3'- cGUGG--CUCGUugUCCAa----GCUUUUGGU- -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 25035 | 0.68 | 0.941942 |
Target: 5'- cGCGCCaguguGAGUGGCGGGUuggcgUUGuuGACCAc -3' miRNA: 3'- -CGUGG-----CUCGUUGUCCA-----AGCuuUUGGU- -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 40341 | 0.68 | 0.931227 |
Target: 5'- aCACCcGGCGucugACGGGUUCGGGcuaaucGACCGc -3' miRNA: 3'- cGUGGcUCGU----UGUCCAAGCUU------UUGGU- -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 6628 | 0.69 | 0.892144 |
Target: 5'- cGCACCGAGaacgAGCAGGaUC--AAACCGg -3' miRNA: 3'- -CGUGGCUCg---UUGUCCaAGcuUUUGGU- -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 27383 | 0.7 | 0.849355 |
Target: 5'- uGUACCGcuucugcgaugggcAGCAACAGGUcUCGGAuguCCGc -3' miRNA: 3'- -CGUGGC--------------UCGUUGUCCA-AGCUUuu-GGU- -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 45141 | 0.74 | 0.667074 |
Target: 5'- gGCAUCGAGCcuGACAGcg-UGAAAGCCAa -3' miRNA: 3'- -CGUGGCUCG--UUGUCcaaGCUUUUGGU- -5' |
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20774 | 5' | -49.3 | NC_004689.1 | + | 19103 | 1.12 | 0.003654 |
Target: 5'- cGCACCGAGCAACAGGUUCGAAAACCAc -3' miRNA: 3'- -CGUGGCUCGUUGUCCAAGCUUUUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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