Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20775 | 3' | -58.4 | NC_004689.1 | + | 7374 | 0.66 | 0.588505 |
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Target: 5'- aGUUGCGCGaauacgcGCCCcggCUGUGGUacauguccgaggcuUGGCg -3' miRNA: 3'- aCAGCGCGUa------UGGGa--GACGCCG--------------ACCG- -5' |
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| 20775 | 3' | -58.4 | NC_004689.1 | + | 65643 | 0.66 | 0.570564 |
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Target: 5'- -uUCGC-CGUACCagaUCgGCuGGCUGGUg -3' miRNA: 3'- acAGCGcGUAUGGg--AGaCG-CCGACCG- -5' |
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| 20775 | 3' | -58.4 | NC_004689.1 | + | 48750 | 0.67 | 0.529973 |
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Target: 5'- -aUCGCGCucgGCCUUCUGCuccGGCguguuguugaagucgGGCc -3' miRNA: 3'- acAGCGCGua-UGGGAGACG---CCGa--------------CCG- -5' |
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| 20775 | 3' | -58.4 | NC_004689.1 | + | 1850 | 0.67 | 0.528945 |
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Target: 5'- uUGgCGCGCugAUCCgaugCUGCGGCcucGGCu -3' miRNA: 3'- -ACaGCGCGuaUGGGa---GACGCCGa--CCG- -5' |
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| 20775 | 3' | -58.4 | NC_004689.1 | + | 224 | 0.7 | 0.394543 |
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Target: 5'- cGUCGUGCuUAUCCgacgcuUCUGCGaaUGGCa -3' miRNA: 3'- aCAGCGCGuAUGGG------AGACGCcgACCG- -5' |
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| 20775 | 3' | -58.4 | NC_004689.1 | + | 31893 | 0.7 | 0.389284 |
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Target: 5'- aUGUCGaUGCcaccggggcccacuUGCCCUgUGCGGCUccGGCc -3' miRNA: 3'- -ACAGC-GCGu-------------AUGGGAgACGCCGA--CCG- -5' |
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| 20775 | 3' | -58.4 | NC_004689.1 | + | 63247 | 0.7 | 0.377195 |
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Target: 5'- aG-CGCGCuacUGCCCUCguccUGUGGCgGGUa -3' miRNA: 3'- aCaGCGCGu--AUGGGAG----ACGCCGaCCG- -5' |
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| 20775 | 3' | -58.4 | NC_004689.1 | + | 64887 | 0.72 | 0.277406 |
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Target: 5'- cGUCaCGCAU-CCCUCUGCGGUgaccugucccuUGcGCa -3' miRNA: 3'- aCAGcGCGUAuGGGAGACGCCG-----------AC-CG- -5' |
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| 20775 | 3' | -58.4 | NC_004689.1 | + | 19597 | 1.11 | 0.000439 |
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Target: 5'- aUGUCGCGCAUACCCUCUGCGGCUGGCu -3' miRNA: 3'- -ACAGCGCGUAUGGGAGACGCCGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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