Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20777 | 3' | -54.3 | NC_004689.1 | + | 11192 | 0.66 | 0.779346 |
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Target: 5'- -gUG-CGCCG-CGAAUUGC-UGAGUAu -3' miRNA: 3'- aaACgGCGGCgGCUUAACGaACUCGU- -5' |
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| 20777 | 3' | -54.3 | NC_004689.1 | + | 52728 | 0.66 | 0.779346 |
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Target: 5'- cUUGCCGUcguagaCGCCGggUg---UGGGCAu -3' miRNA: 3'- aAACGGCG------GCGGCuuAacgaACUCGU- -5' |
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| 20777 | 3' | -54.3 | NC_004689.1 | + | 53116 | 0.66 | 0.779346 |
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Target: 5'- -gUGCCGUCGUCcagcgucuucguGAAcugacgaUGCUUGAGCGa -3' miRNA: 3'- aaACGGCGGCGG------------CUUa------ACGAACUCGU- -5' |
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| 20777 | 3' | -54.3 | NC_004689.1 | + | 37375 | 0.66 | 0.757761 |
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Target: 5'- cUUGCCGCUGCgGAAaacgaccacgGCUUcgccgucGAGCAa -3' miRNA: 3'- aAACGGCGGCGgCUUaa--------CGAA-------CUCGU- -5' |
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| 20777 | 3' | -54.3 | NC_004689.1 | + | 40784 | 0.68 | 0.661132 |
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Target: 5'- --gGCagauaGCCGCUGuAGUggcucgGCUUGAGCGg -3' miRNA: 3'- aaaCGg----CGGCGGC-UUAa-----CGAACUCGU- -5' |
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| 20777 | 3' | -54.3 | NC_004689.1 | + | 70080 | 0.68 | 0.627659 |
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Target: 5'- --aGCCGUCGUgUGAAUUGCggagacgUGAGCu -3' miRNA: 3'- aaaCGGCGGCG-GCUUAACGa------ACUCGu -5' |
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| 20777 | 3' | -54.3 | NC_004689.1 | + | 40837 | 0.72 | 0.425886 |
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Target: 5'- --cGuuGCCGUCGAGUgccUGCcagUUGAGCAg -3' miRNA: 3'- aaaCggCGGCGGCUUA---ACG---AACUCGU- -5' |
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| 20777 | 3' | -54.3 | NC_004689.1 | + | 20111 | 0.89 | 0.034427 |
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Target: 5'- cUUUGCCGCCGCCGAAUUcgUUGAGCAg -3' miRNA: 3'- -AAACGGCGGCGGCUUAAcgAACUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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