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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20777 | 5' | -51.8 | NC_004689.1 | + | 51530 | 0.66 | 0.924633 |
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Target: 5'- cUGUGCgauUGCAGAcgGUCUacUGuGCGGUAGCc -3' miRNA: 3'- -ACACG---ACGUUU--UAGA--GC-CGCCGUUGc -5' |
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| 20777 | 5' | -51.8 | NC_004689.1 | + | 13763 | 0.66 | 0.918566 |
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Target: 5'- --cGCUGCGGc--CUCGG-GGUGACGg -3' miRNA: 3'- acaCGACGUUuuaGAGCCgCCGUUGC- -5' |
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| 20777 | 5' | -51.8 | NC_004689.1 | + | 3985 | 0.66 | 0.905581 |
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Target: 5'- cGUGCUGacgaAAGAUCgucuccaauguUCGGCuugcuucuggGGCAGCa -3' miRNA: 3'- aCACGACg---UUUUAG-----------AGCCG----------CCGUUGc -5' |
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| 20777 | 5' | -51.8 | NC_004689.1 | + | 20874 | 0.66 | 0.898667 |
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Target: 5'- aUGcGCUGCAA---UUCGGCaGCGGCu -3' miRNA: 3'- -ACaCGACGUUuuaGAGCCGcCGUUGc -5' |
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| 20777 | 5' | -51.8 | NC_004689.1 | + | 22889 | 0.67 | 0.868296 |
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Target: 5'- -uUGCUugaGCAAu-UCuUCGGCGGCuGCGa -3' miRNA: 3'- acACGA---CGUUuuAG-AGCCGCCGuUGC- -5' |
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| 20777 | 5' | -51.8 | NC_004689.1 | + | 46352 | 0.68 | 0.815329 |
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Target: 5'- -uUGcCUGCGAAG-CUCGGCGuGCGuucgGCGa -3' miRNA: 3'- acAC-GACGUUUUaGAGCCGC-CGU----UGC- -5' |
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| 20777 | 5' | -51.8 | NC_004689.1 | + | 33695 | 0.69 | 0.795979 |
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Target: 5'- --aGCUGCAcAAgcugaUCGGCuccGGCAGCGg -3' miRNA: 3'- acaCGACGUuUUag---AGCCG---CCGUUGC- -5' |
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| 20777 | 5' | -51.8 | NC_004689.1 | + | 20076 | 1.09 | 0.002761 |
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Target: 5'- aUGUGCUGCAAAAUCUCGGCGGCAACGc -3' miRNA: 3'- -ACACGACGUUUUAGAGCCGCCGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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