Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20778 | 3' | -57.4 | NC_004689.1 | + | 68052 | 0.66 | 0.660523 |
Target: 5'- --cGCCUUuCGGCCuuCUGGUgUGAGg -3' miRNA: 3'- cuaCGGGAuGCCGGuuGACCGgGUUC- -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 48764 | 0.66 | 0.649735 |
Target: 5'- cGUGCCUUGCGGgCAucgcGCUcGGCCUu-- -3' miRNA: 3'- cUACGGGAUGCCgGU----UGA-CCGGGuuc -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 65517 | 0.66 | 0.649735 |
Target: 5'- --gGCCUccacgucGCGGCCAGucucuUUGGCCCAGu -3' miRNA: 3'- cuaCGGGa------UGCCGGUU-----GACCGGGUUc -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 51271 | 0.66 | 0.617314 |
Target: 5'- -cUGCUgguucGCGGCCAACgUGGUCCAGu -3' miRNA: 3'- cuACGGga---UGCCGGUUG-ACCGGGUUc -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 48883 | 0.66 | 0.60652 |
Target: 5'- uGUGCCUcggcggcacgUGCGGCCAuccGCUGaGCCUg-- -3' miRNA: 3'- cUACGGG----------AUGCCGGU---UGAC-CGGGuuc -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 11421 | 0.67 | 0.56364 |
Target: 5'- uGAUGCCCacCGGCagaacgCggUUGGCCUGAGu -3' miRNA: 3'- -CUACGGGauGCCG------GuuGACCGGGUUC- -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 17104 | 0.67 | 0.542489 |
Target: 5'- --gGCCCUGCGGUgaGGCcGGUCUGAGu -3' miRNA: 3'- cuaCGGGAUGCCGg-UUGaCCGGGUUC- -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 31870 | 0.67 | 0.542489 |
Target: 5'- -uUGCCCUguGCGGCU--CcGGCCCAu- -3' miRNA: 3'- cuACGGGA--UGCCGGuuGaCCGGGUuc -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 49723 | 0.7 | 0.404419 |
Target: 5'- -uUGCCCUugGGCgGugUguaguauucGGCCCAc- -3' miRNA: 3'- cuACGGGAugCCGgUugA---------CCGGGUuc -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 27349 | 0.71 | 0.369189 |
Target: 5'- gGAUGUCCgcaaugaacACGGCCAACUGcugcaacgccuGUCCGAGg -3' miRNA: 3'- -CUACGGGa--------UGCCGGUUGAC-----------CGGGUUC- -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 35223 | 0.73 | 0.265788 |
Target: 5'- --aGCUCUGCGGCCAAUUGGauugcaggcgaugauCCCAGa -3' miRNA: 3'- cuaCGGGAUGCCGGUUGACC---------------GGGUUc -5' |
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20778 | 3' | -57.4 | NC_004689.1 | + | 20293 | 1.09 | 0.000739 |
Target: 5'- gGAUGCCCUACGGCCAACUGGCCCAAGg -3' miRNA: 3'- -CUACGGGAUGCCGGUUGACCGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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