Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20779 | 5' | -54.5 | NC_004689.1 | + | 14101 | 0.66 | 0.838009 |
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Target: 5'- aCCGGaguCCA-GCCACGcaccgauugcaauGUCGGUGAUGc -3' miRNA: 3'- aGGCU---GGUaCGGUGC-------------CAGUCACUACu -5' |
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| 20779 | 5' | -54.5 | NC_004689.1 | + | 23621 | 0.66 | 0.830087 |
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Target: 5'- uUUCaACCAUGCCACGuucGcCGGUGAUcGAa -3' miRNA: 3'- -AGGcUGGUACGGUGC---CaGUCACUA-CU- -5' |
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| 20779 | 5' | -54.5 | NC_004689.1 | + | 54868 | 0.66 | 0.802534 |
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Target: 5'- aCCGACCGcacagggauucUGCgACGG-CGGUGG-GAa -3' miRNA: 3'- aGGCUGGU-----------ACGgUGCCaGUCACUaCU- -5' |
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| 20779 | 5' | -54.5 | NC_004689.1 | + | 2278 | 0.68 | 0.701019 |
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Target: 5'- --gGACCGUGuucCCACGacaaggauugcGUCAGUGGUGAu -3' miRNA: 3'- aggCUGGUAC---GGUGC-----------CAGUCACUACU- -5' |
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| 20779 | 5' | -54.5 | NC_004689.1 | + | 16547 | 0.68 | 0.679581 |
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Target: 5'- aCCGAugcaCCAcgGCCACGcGU-GGUGAUGAg -3' miRNA: 3'- aGGCU----GGUa-CGGUGC-CAgUCACUACU- -5' |
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| 20779 | 5' | -54.5 | NC_004689.1 | + | 25121 | 0.69 | 0.668791 |
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Target: 5'- gUCCGGUCGcUGCCAUGGUCAccGUGAg-- -3' miRNA: 3'- -AGGCUGGU-ACGGUGCCAGU--CACUacu -5' |
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| 20779 | 5' | -54.5 | NC_004689.1 | + | 41099 | 0.73 | 0.401142 |
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Target: 5'- gCCGAUgAUGuUCGCGGUCuccGUGAUGAa -3' miRNA: 3'- aGGCUGgUAC-GGUGCCAGu--CACUACU- -5' |
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| 20779 | 5' | -54.5 | NC_004689.1 | + | 20507 | 1.1 | 0.001479 |
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Target: 5'- uUCCGACCAUGCCACGGUCAGUGAUGAa -3' miRNA: 3'- -AGGCUGGUACGGUGCCAGUCACUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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