Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2078 | 3' | -53.7 | NC_001348.1 | + | 84589 | 0.66 | 0.953928 |
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Target: 5'- cGAUCGGGAUUG--GAUaGGAUCGAc- -3' miRNA: 3'- cCUAGCCCUGGCgaCUA-CCUAGCUau -5' |
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| 2078 | 3' | -53.7 | NC_001348.1 | + | 20926 | 0.66 | 0.944903 |
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Target: 5'- gGGAUCGGGcuuucgggaagcgGCCGa-GGUGGggCGAc- -3' miRNA: 3'- -CCUAGCCC-------------UGGCgaCUACCuaGCUau -5' |
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| 2078 | 3' | -53.7 | NC_001348.1 | + | 20657 | 0.66 | 0.940704 |
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Target: 5'- -uGUUGGGugUugGCUGcgGGAUCGGg- -3' miRNA: 3'- ccUAGCCCugG--CGACuaCCUAGCUau -5' |
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| 2078 | 3' | -53.7 | NC_001348.1 | + | 70063 | 0.67 | 0.901133 |
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Target: 5'- gGGAUCGGGG--GC-GGUGGAUCGcgAc -3' miRNA: 3'- -CCUAGCCCUggCGaCUACCUAGCuaU- -5' |
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| 2078 | 3' | -53.7 | NC_001348.1 | + | 90250 | 0.68 | 0.894487 |
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Target: 5'- gGGAUCGuGGCCGCgGAUaaacGGggCGGUAa -3' miRNA: 3'- -CCUAGCcCUGGCGaCUA----CCuaGCUAU- -5' |
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| 2078 | 3' | -53.7 | NC_001348.1 | + | 107755 | 1.09 | 0.003776 |
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Target: 5'- cGGAUCGGGACCGCUGAUGGAUCGAUAu -3' miRNA: 3'- -CCUAGCCCUGGCGACUACCUAGCUAU- -5' |
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| 2078 | 3' | -53.7 | NC_001348.1 | + | 122037 | 1.09 | 0.003776 |
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Target: 5'- cGGAUCGGGACCGCUGAUGGAUCGAUAu -3' miRNA: 3'- -CCUAGCCCUGGCGACUACCUAGCUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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