Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20781 | 3' | -53.5 | NC_004689.1 | + | 36529 | 0.66 | 0.845538 |
Target: 5'- -aCGCGCGUGCGCgccCGAUacCGcGCGGg -3' miRNA: 3'- caGUGCGUACGCGac-GUUAa-GC-CGUC- -5' |
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20781 | 3' | -53.5 | NC_004689.1 | + | 65580 | 0.66 | 0.817454 |
Target: 5'- -cCGCGCGUGCGCUucguccaGCGuggagaacaUCGGCGu -3' miRNA: 3'- caGUGCGUACGCGA-------CGUua-------AGCCGUc -5' |
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20781 | 3' | -53.5 | NC_004689.1 | + | 20083 | 0.66 | 0.80893 |
Target: 5'- aUCGagaAUGUGCUGCAAaaucUCGGCGGc -3' miRNA: 3'- cAGUgcgUACGCGACGUUa---AGCCGUC- -5' |
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20781 | 3' | -53.5 | NC_004689.1 | + | 13776 | 0.67 | 0.799279 |
Target: 5'- gGUUACGUGccUGCGCUGCGGccUCGGgGu -3' miRNA: 3'- -CAGUGCGU--ACGCGACGUUa-AGCCgUc -5' |
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20781 | 3' | -53.5 | NC_004689.1 | + | 62752 | 0.69 | 0.650693 |
Target: 5'- cUCGCGCAUGCGUaugagaGCcagUCGGCGu -3' miRNA: 3'- cAGUGCGUACGCGa-----CGuuaAGCCGUc -5' |
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20781 | 3' | -53.5 | NC_004689.1 | + | 20882 | 1.08 | 0.001897 |
Target: 5'- cGUCACGCAUGCGCUGCAAUUCGGCAGc -3' miRNA: 3'- -CAGUGCGUACGCGACGUUAAGCCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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