miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20781 3' -53.5 NC_004689.1 + 36529 0.66 0.845538
Target:  5'- -aCGCGCGUGCGCgccCGAUacCGcGCGGg -3'
miRNA:   3'- caGUGCGUACGCGac-GUUAa-GC-CGUC- -5'
20781 3' -53.5 NC_004689.1 + 65580 0.66 0.817454
Target:  5'- -cCGCGCGUGCGCUucguccaGCGuggagaacaUCGGCGu -3'
miRNA:   3'- caGUGCGUACGCGA-------CGUua-------AGCCGUc -5'
20781 3' -53.5 NC_004689.1 + 20083 0.66 0.80893
Target:  5'- aUCGagaAUGUGCUGCAAaaucUCGGCGGc -3'
miRNA:   3'- cAGUgcgUACGCGACGUUa---AGCCGUC- -5'
20781 3' -53.5 NC_004689.1 + 13776 0.67 0.799279
Target:  5'- gGUUACGUGccUGCGCUGCGGccUCGGgGu -3'
miRNA:   3'- -CAGUGCGU--ACGCGACGUUa-AGCCgUc -5'
20781 3' -53.5 NC_004689.1 + 62752 0.69 0.650693
Target:  5'- cUCGCGCAUGCGUaugagaGCcagUCGGCGu -3'
miRNA:   3'- cAGUGCGUACGCGa-----CGuuaAGCCGUc -5'
20781 3' -53.5 NC_004689.1 + 20882 1.08 0.001897
Target:  5'- cGUCACGCAUGCGCUGCAAUUCGGCAGc -3'
miRNA:   3'- -CAGUGCGUACGCGACGUUAAGCCGUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.