Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20781 | 5' | -57.1 | NC_004689.1 | + | 53724 | 0.66 | 0.691336 |
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Target: 5'- --gCGCCGUCACUG-AG-UGUGCAGg -3' miRNA: 3'- aagGUGGCAGUGGCcUCaACGCGUCg -5' |
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| 20781 | 5' | -57.1 | NC_004689.1 | + | 52455 | 0.66 | 0.669992 |
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Target: 5'- cUCUACCGcuuUCACCGGcgcuggacuAGccUGCGCGGg -3' miRNA: 3'- aAGGUGGC---AGUGGCC---------UCa-ACGCGUCg -5' |
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| 20781 | 5' | -57.1 | NC_004689.1 | + | 30388 | 0.66 | 0.659264 |
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Target: 5'- gUCCGuugauCCGUgACCGGAGUuUGUGgAGg -3' miRNA: 3'- aAGGU-----GGCAgUGGCCUCA-ACGCgUCg -5' |
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| 20781 | 5' | -57.1 | NC_004689.1 | + | 36322 | 0.66 | 0.659264 |
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Target: 5'- -gCCugUGUCACgCGGGGaaucgcgccuguUUGCGC-GCg -3' miRNA: 3'- aaGGugGCAGUG-GCCUC------------AACGCGuCG- -5' |
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| 20781 | 5' | -57.1 | NC_004689.1 | + | 13328 | 0.67 | 0.616205 |
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Target: 5'- cUCCGgUGgcUCACCGGccuGUUcGUGCGGCa -3' miRNA: 3'- aAGGUgGC--AGUGGCCu--CAA-CGCGUCG- -5' |
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| 20781 | 5' | -57.1 | NC_004689.1 | + | 51289 | 0.68 | 0.531235 |
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Target: 5'- -cCCACCGguugaCACCGGcugcuGGUU-CGCGGCc -3' miRNA: 3'- aaGGUGGCa----GUGGCC-----UCAAcGCGUCG- -5' |
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| 20781 | 5' | -57.1 | NC_004689.1 | + | 1893 | 0.73 | 0.283755 |
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Target: 5'- -cCCACCGaCACgCGGGccGUUGgGCAGCu -3' miRNA: 3'- aaGGUGGCaGUG-GCCU--CAACgCGUCG- -5' |
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| 20781 | 5' | -57.1 | NC_004689.1 | + | 53172 | 0.76 | 0.17882 |
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Target: 5'- aUCCACCaucgCGCCGGAGgaaCGCGGCg -3' miRNA: 3'- aAGGUGGca--GUGGCCUCaacGCGUCG- -5' |
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| 20781 | 5' | -57.1 | NC_004689.1 | + | 25110 | 0.76 | 0.17882 |
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Target: 5'- -gCCAUgGUCACCGuGAGguuUGCGCGGUg -3' miRNA: 3'- aaGGUGgCAGUGGC-CUCa--ACGCGUCG- -5' |
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| 20781 | 5' | -57.1 | NC_004689.1 | + | 20847 | 1.1 | 0.000734 |
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Target: 5'- uUUCCACCGUCACCGGAGUUGCGCAGCg -3' miRNA: 3'- -AAGGUGGCAGUGGCCUCAACGCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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