Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20782 | 3' | -49.2 | NC_004689.1 | + | 15644 | 0.66 | 0.98451 |
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Target: 5'- uUCGGUCgUACAacacgCUGAaugUGGCGUuucgUGCg -3' miRNA: 3'- cAGCCAG-GUGUa----GACUa--ACUGCA----ACG- -5' |
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| 20782 | 3' | -49.2 | NC_004689.1 | + | 10615 | 0.66 | 0.982437 |
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Target: 5'- -gCGGUCUGCGgagcgcCUGccggaGACGUUGCg -3' miRNA: 3'- caGCCAGGUGUa-----GACuaa--CUGCAACG- -5' |
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| 20782 | 3' | -49.2 | NC_004689.1 | + | 20904 | 0.66 | 0.980162 |
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Target: 5'- aUCaG-CCGCAUCUGGUUGAaaaCGUcacgcaUGCg -3' miRNA: 3'- cAGcCaGGUGUAGACUAACU---GCA------ACG- -5' |
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| 20782 | 3' | -49.2 | NC_004689.1 | + | 67115 | 0.67 | 0.961708 |
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Target: 5'- uUCGGUgCCACGUC-GAgcUUGACGa-GCa -3' miRNA: 3'- cAGCCA-GGUGUAGaCU--AACUGCaaCG- -5' |
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| 20782 | 3' | -49.2 | NC_004689.1 | + | 35434 | 0.68 | 0.953524 |
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Target: 5'- --gGGUCCgcuGCAUUgcgGAgcGGCGUUGCc -3' miRNA: 3'- cagCCAGG---UGUAGa--CUaaCUGCAACG- -5' |
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| 20782 | 3' | -49.2 | NC_004689.1 | + | 64261 | 0.68 | 0.953524 |
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Target: 5'- -gCGGUagGCGUCaggGAUcGACGUUGCc -3' miRNA: 3'- caGCCAggUGUAGa--CUAaCUGCAACG- -5' |
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| 20782 | 3' | -49.2 | NC_004689.1 | + | 8135 | 0.68 | 0.933809 |
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Target: 5'- --aGGUCCAC----GAcUGACGUUGCg -3' miRNA: 3'- cagCCAGGUGuagaCUaACUGCAACG- -5' |
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| 20782 | 3' | -49.2 | NC_004689.1 | + | 29913 | 0.7 | 0.895621 |
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Target: 5'- cGUCGGggaaCCACAUCagGAUUG-CcUUGCa -3' miRNA: 3'- -CAGCCa---GGUGUAGa-CUAACuGcAACG- -5' |
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| 20782 | 3' | -49.2 | NC_004689.1 | + | 22165 | 0.76 | 0.572006 |
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Target: 5'- aGUCGGUCCACuGUCggGAgc-ACGUUGCc -3' miRNA: 3'- -CAGCCAGGUG-UAGa-CUaacUGCAACG- -5' |
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| 20782 | 3' | -49.2 | NC_004689.1 | + | 21271 | 1.12 | 0.003998 |
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Target: 5'- uGUCGGUCCACAUCUGAUUGACGUUGCc -3' miRNA: 3'- -CAGCCAGGUGUAGACUAACUGCAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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