Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20784 | 3' | -52.2 | NC_004689.1 | + | 67603 | 0.66 | 0.881266 |
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Target: 5'- cGGggGauucuucuuuCGAGCCGgaagCGGCaGCGUUCc -3' miRNA: 3'- -UCuuC----------GUUCGGUaa--GCCGaCGCAAGc -5' |
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| 20784 | 3' | -52.2 | NC_004689.1 | + | 3671 | 0.67 | 0.86541 |
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Target: 5'- cGAAGUcAGUgaAUUCGGCUGCGauUUCc -3' miRNA: 3'- uCUUCGuUCGg-UAAGCCGACGC--AAGc -5' |
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| 20784 | 3' | -52.2 | NC_004689.1 | + | 401 | 0.68 | 0.830773 |
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Target: 5'- --cGGUAcGCaCAUUCGGCUGCGggaauUUCGc -3' miRNA: 3'- ucuUCGUuCG-GUAAGCCGACGC-----AAGC- -5' |
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| 20784 | 3' | -52.2 | NC_004689.1 | + | 67830 | 0.7 | 0.709103 |
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Target: 5'- aGGAAGCGGGCCAU---GC-GCGUUCu -3' miRNA: 3'- -UCUUCGUUCGGUAagcCGaCGCAAGc -5' |
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| 20784 | 3' | -52.2 | NC_004689.1 | + | 485 | 0.71 | 0.620496 |
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Target: 5'- cAGguGCGGGCCGUcCGGUUugcccaugagcGCGUUCGg -3' miRNA: 3'- -UCuuCGUUCGGUAaGCCGA-----------CGCAAGC- -5' |
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| 20784 | 3' | -52.2 | NC_004689.1 | + | 23156 | 1.09 | 0.002622 |
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Target: 5'- gAGAAGCAAGCCAUUCGGCUGCGUUCGc -3' miRNA: 3'- -UCUUCGUUCGGUAAGCCGACGCAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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