Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20784 | 5' | -60.5 | NC_004689.1 | + | 22229 | 0.66 | 0.514524 |
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Target: 5'- -gCGAcCGGCCAgCGGacccuucuUCGG-CGGCg -3' miRNA: 3'- uaGCUuGCCGGUgGCC--------AGCCuGCCGa -5' |
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| 20784 | 5' | -60.5 | NC_004689.1 | + | 10621 | 0.66 | 0.494777 |
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Target: 5'- uGUUGGGCGGUCugcggagcgccuGCCGGagacguugCGGGCGGUg -3' miRNA: 3'- -UAGCUUGCCGG------------UGGCCa-------GCCUGCCGa -5' |
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| 20784 | 5' | -60.5 | NC_004689.1 | + | 12865 | 0.68 | 0.410731 |
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Target: 5'- gGUCGAAcCGGCCACCGGaacCGaugaGGCa -3' miRNA: 3'- -UAGCUU-GCCGGUGGCCa--GCcug-CCGa -5' |
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| 20784 | 5' | -60.5 | NC_004689.1 | + | 40296 | 0.68 | 0.409847 |
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Target: 5'- --aGAACGucuGCgACCGGaccuugaUCGGACGGCg -3' miRNA: 3'- uagCUUGC---CGgUGGCC-------AGCCUGCCGa -5' |
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| 20784 | 5' | -60.5 | NC_004689.1 | + | 23122 | 1.06 | 0.000796 |
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Target: 5'- cAUCGAACGGCCACCGGUCGGACGGCUg -3' miRNA: 3'- -UAGCUUGCCGGUGGCCAGCCUGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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