Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20788 | 5' | -53.2 | NC_004689.1 | + | 14109 | 0.66 | 0.896726 |
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Target: 5'- uGGGAcagaccggaguccaGCCACGcACCG-AUUGCaAUGUCg -3' miRNA: 3'- -CCCU--------------CGGUGC-UGGCuUAGUGcUACAG- -5' |
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| 20788 | 5' | -53.2 | NC_004689.1 | + | 23499 | 0.66 | 0.891808 |
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Target: 5'- aGGGAuGCCGCGACCugcuGAUCGCu-UGa- -3' miRNA: 3'- -CCCU-CGGUGCUGGc---UUAGUGcuACag -5' |
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| 20788 | 5' | -53.2 | NC_004689.1 | + | 5019 | 0.66 | 0.881596 |
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Target: 5'- aGGGAucGUCAgGGCUGcggaaauccugCACGAUGUCa -3' miRNA: 3'- -CCCU--CGGUgCUGGCuua--------GUGCUACAG- -5' |
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| 20788 | 5' | -53.2 | NC_004689.1 | + | 66806 | 0.68 | 0.779975 |
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Target: 5'- ---uGCCccaccauuugaGCGugCGGAUCAUGGUGUCu -3' miRNA: 3'- cccuCGG-----------UGCugGCUUAGUGCUACAG- -5' |
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| 20788 | 5' | -53.2 | NC_004689.1 | + | 11087 | 0.69 | 0.748768 |
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Target: 5'- cGGGAGCCGCuGGCgGggUCugcugcuGCGGguUGUUc -3' miRNA: 3'- -CCCUCGGUG-CUGgCuuAG-------UGCU--ACAG- -5' |
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| 20788 | 5' | -53.2 | NC_004689.1 | + | 60059 | 0.7 | 0.697302 |
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Target: 5'- cGGGcugcaugucAGCCAUGACCGAuccccUCGCacAUGUCg -3' miRNA: 3'- -CCC---------UCGGUGCUGGCUu----AGUGc-UACAG- -5' |
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| 20788 | 5' | -53.2 | NC_004689.1 | + | 70407 | 0.7 | 0.674717 |
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Target: 5'- cGGGuGCCACGACCauggucggcucauGAucuugacugccGUC-CGGUGUCu -3' miRNA: 3'- -CCCuCGGUGCUGG-------------CU-----------UAGuGCUACAG- -5' |
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| 20788 | 5' | -53.2 | NC_004689.1 | + | 25955 | 1.12 | 0.001632 |
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Target: 5'- gGGGAGCCACGACCGAAUCACGAUGUCu -3' miRNA: 3'- -CCCUCGGUGCUGGCUUAGUGCUACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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