Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20791 | 5' | -60 | NC_004689.1 | + | 23297 | 0.66 | 0.528589 |
Target: 5'- aGCCAGUacgacCGCCcaaCGCUGCGGCUg -3' miRNA: 3'- -UGGUCAguc--GCGGca-GCGACGCUGGu -5' |
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20791 | 5' | -60 | NC_004689.1 | + | 37146 | 0.66 | 0.528589 |
Target: 5'- aGCuCAGUcCAGCGCCGgu-CUGCGuCCc -3' miRNA: 3'- -UG-GUCA-GUCGCGGCagcGACGCuGGu -5' |
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20791 | 5' | -60 | NC_004689.1 | + | 62734 | 0.66 | 0.518468 |
Target: 5'- aGCCAGUCGGCGUU-UCuCaGCGGCCu -3' miRNA: 3'- -UGGUCAGUCGCGGcAGcGaCGCUGGu -5' |
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20791 | 5' | -60 | NC_004689.1 | + | 63413 | 0.68 | 0.41329 |
Target: 5'- aGCCAGcCAGCGaCCGUUGC-GCcgGAUCGg -3' miRNA: 3'- -UGGUCaGUCGC-GGCAGCGaCG--CUGGU- -5' |
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20791 | 5' | -60 | NC_004689.1 | + | 44903 | 0.69 | 0.33769 |
Target: 5'- aGCUuGUCuGUGUCGUCGCUGCGGa-- -3' miRNA: 3'- -UGGuCAGuCGCGGCAGCGACGCUggu -5' |
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20791 | 5' | -60 | NC_004689.1 | + | 57020 | 0.78 | 0.088685 |
Target: 5'- gGCCGGgacggcUCAGCGCCGU-GCaUGCGGCCAa -3' miRNA: 3'- -UGGUC------AGUCGCGGCAgCG-ACGCUGGU- -5' |
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20791 | 5' | -60 | NC_004689.1 | + | 30255 | 1.08 | 0.000566 |
Target: 5'- cACCAGUCAGCGCCGUCGCUGCGACCAa -3' miRNA: 3'- -UGGUCAGUCGCGGCAGCGACGCUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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