Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20792 | 3' | -50.7 | NC_004689.1 | + | 9029 | 0.67 | 0.930819 |
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Target: 5'- --cGCCGccUUGCCGCCUUUcgccGCgcggGCCGc -3' miRNA: 3'- acuUGGC--AACGGCGGAAA----UGa---UGGUc -5' |
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| 20792 | 3' | -50.7 | NC_004689.1 | + | 1842 | 0.68 | 0.876573 |
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Target: 5'- cUGAuCCGaugcUGCgGCCUcgGCUGCCAc -3' miRNA: 3'- -ACUuGGCa---ACGgCGGAaaUGAUGGUc -5' |
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| 20792 | 3' | -50.7 | NC_004689.1 | + | 21701 | 0.68 | 0.876573 |
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Target: 5'- gGAGCCGgUGCC-CCag-GCUGCaCAGg -3' miRNA: 3'- aCUUGGCaACGGcGGaaaUGAUG-GUC- -5' |
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| 20792 | 3' | -50.7 | NC_004689.1 | + | 51665 | 0.68 | 0.868557 |
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Target: 5'- gGAugCGuUUGCCGCCUUUuu--UCAGa -3' miRNA: 3'- aCUugGC-AACGGCGGAAAugauGGUC- -5' |
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| 20792 | 3' | -50.7 | NC_004689.1 | + | 33606 | 0.69 | 0.843006 |
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Target: 5'- cGcACCGgccacGCCGCCUgcUACUGCCu- -3' miRNA: 3'- aCuUGGCaa---CGGCGGAa-AUGAUGGuc -5' |
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| 20792 | 3' | -50.7 | NC_004689.1 | + | 40846 | 0.7 | 0.815381 |
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Target: 5'- cGAucuucaCGUUGCCGUCgagUGcCUGCCAGu -3' miRNA: 3'- aCUug----GCAACGGCGGaa-AU-GAUGGUC- -5' |
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| 20792 | 3' | -50.7 | NC_004689.1 | + | 14521 | 0.7 | 0.805758 |
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Target: 5'- -uGACC-UUGCCGCCUUgaucgGCacccgGCCAGa -3' miRNA: 3'- acUUGGcAACGGCGGAAa----UGa----UGGUC- -5' |
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| 20792 | 3' | -50.7 | NC_004689.1 | + | 39469 | 0.71 | 0.712026 |
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Target: 5'- -uGGCCGgUGCCuGUCggUGCUGCCAGu -3' miRNA: 3'- acUUGGCaACGG-CGGaaAUGAUGGUC- -5' |
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| 20792 | 3' | -50.7 | NC_004689.1 | + | 21619 | 0.72 | 0.678869 |
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Target: 5'- gGAACUGUUGUaggguCGCCaacGCUGCCGGa -3' miRNA: 3'- aCUUGGCAACG-----GCGGaaaUGAUGGUC- -5' |
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| 20792 | 3' | -50.7 | NC_004689.1 | + | 30902 | 1.03 | 0.008806 |
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Target: 5'- cUGAACCGUUGCCGCCUUUACUACCGc -3' miRNA: 3'- -ACUUGGCAACGGCGGAAAUGAUGGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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