Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20794 | 3' | -49.2 | NC_004689.1 | + | 2435 | 0.66 | 0.970883 |
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Target: 5'- cGGUCGUGUgguGCC-CAGugguaGCCGAUCa -3' miRNA: 3'- aCUAGCAUAacuUGGcGUC-----CGGUUAG- -5' |
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| 20794 | 3' | -49.2 | NC_004689.1 | + | 14058 | 0.66 | 0.970883 |
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Target: 5'- aGAUCGU-UUGcuUCGUcGGCCAGUUg -3' miRNA: 3'- aCUAGCAuAACuuGGCGuCCGGUUAG- -5' |
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| 20794 | 3' | -49.2 | NC_004689.1 | + | 12238 | 0.67 | 0.960151 |
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Target: 5'- -uGUCGUAgu--GCCGCAGGUCGu-- -3' miRNA: 3'- acUAGCAUaacuUGGCGUCCGGUuag -5' |
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| 20794 | 3' | -49.2 | NC_004689.1 | + | 68562 | 0.68 | 0.94199 |
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Target: 5'- cUGGUCGUcggUGGAcCCGCAcucuaggucgguGGCCGGUa -3' miRNA: 3'- -ACUAGCAua-ACUU-GGCGU------------CCGGUUAg -5' |
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| 20794 | 3' | -49.2 | NC_004689.1 | + | 41000 | 0.7 | 0.868021 |
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Target: 5'- cUGAUCGaGUUG-GCCGguGGCCc--- -3' miRNA: 3'- -ACUAGCaUAACuUGGCguCCGGuuag -5' |
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| 20794 | 3' | -49.2 | NC_004689.1 | + | 28831 | 0.73 | 0.697642 |
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Target: 5'- gGGUCGccu---GCUGCGGGCCAAUCc -3' miRNA: 3'- aCUAGCauaacuUGGCGUCCGGUUAG- -5' |
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| 20794 | 3' | -49.2 | NC_004689.1 | + | 31775 | 1.1 | 0.004525 |
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Target: 5'- gUGAUCGUAUUGAACCGCAGGCCAAUCc -3' miRNA: 3'- -ACUAGCAUAACUUGGCGUCCGGUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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