Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20794 | 5' | -58 | NC_004689.1 | + | 29805 | 0.66 | 0.687523 |
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Target: 5'- ----cCGGCaCCGGgagUUCCACCGuuGg -3' miRNA: 3'- cgccaGCCG-GGCCaa-AAGGUGGUggC- -5' |
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| 20794 | 5' | -58 | NC_004689.1 | + | 11959 | 0.67 | 0.624806 |
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Target: 5'- gGCGuacCGGUgCGGUa--CCGCCGCCGa -3' miRNA: 3'- -CGCca-GCCGgGCCAaaaGGUGGUGGC- -5' |
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| 20794 | 5' | -58 | NC_004689.1 | + | 5266 | 0.67 | 0.582989 |
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Target: 5'- uGCGGU-GGCuuG---UUUCGCCACCGg -3' miRNA: 3'- -CGCCAgCCGggCcaaAAGGUGGUGGC- -5' |
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| 20794 | 5' | -58 | NC_004689.1 | + | 30541 | 0.68 | 0.549938 |
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Target: 5'- cCGGUC-GCCCaGUUUccagccgcaccaCCACCACCGg -3' miRNA: 3'- cGCCAGcCGGGcCAAAa-----------GGUGGUGGC- -5' |
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| 20794 | 5' | -58 | NC_004689.1 | + | 11403 | 0.68 | 0.541761 |
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Target: 5'- cGCGGUUGGCCUGaGUggcgagaUUCACgGCUGc -3' miRNA: 3'- -CGCCAGCCGGGC-CAaa-----AGGUGgUGGC- -5' |
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| 20794 | 5' | -58 | NC_004689.1 | + | 27091 | 0.68 | 0.531597 |
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Target: 5'- cGaaGUCaaGUCCGGUgucUCCACCACCGc -3' miRNA: 3'- -CgcCAGc-CGGGCCAaa-AGGUGGUGGC- -5' |
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| 20794 | 5' | -58 | NC_004689.1 | + | 18594 | 0.7 | 0.453235 |
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Target: 5'- cGCGGUaccccCGGCCCGGUggaCgGCCcuugucagACCGa -3' miRNA: 3'- -CGCCA-----GCCGGGCCAaaaGgUGG--------UGGC- -5' |
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| 20794 | 5' | -58 | NC_004689.1 | + | 53918 | 0.71 | 0.390122 |
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Target: 5'- uCGGUCGGCUCGGgca-CCggguagaacacgGCCACCa -3' miRNA: 3'- cGCCAGCCGGGCCaaaaGG------------UGGUGGc -5' |
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| 20794 | 5' | -58 | NC_004689.1 | + | 40768 | 0.72 | 0.348705 |
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Target: 5'- aGUGGcUCGGCUUgagcgGGUUUUCCAgCCGCCc -3' miRNA: 3'- -CGCC-AGCCGGG-----CCAAAAGGU-GGUGGc -5' |
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| 20794 | 5' | -58 | NC_004689.1 | + | 31741 | 1.1 | 0.000809 |
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Target: 5'- uGCGGUCGGCCCGGUUUUCCACCACCGu -3' miRNA: 3'- -CGCCAGCCGGGCCAAAAGGUGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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