Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 20795 | 5' | -52.6 | NC_004689.1 | + | 44887 | 0.67 | 0.860938 |
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Target: 5'- cGCUGCGgaGCuUGCGCggauCGGGCCUgccggUCACcGCu -3' miRNA: 3'- -CGACGU--UG-ACGUG----GUCUGGA-----AGUG-CG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 3740 | 0.66 | 0.891974 |
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Target: 5'- cCUGCGACggGUugUAaACCagUUCGCGCa -3' miRNA: 3'- cGACGUUGa-CGugGUcUGG--AAGUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 31586 | 0.66 | 0.884602 |
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Target: 5'- -aUGCAACUGggagGCCAGACCca-GCGa -3' miRNA: 3'- cgACGUUGACg---UGGUCUGGaagUGCg -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 39190 | 0.66 | 0.884602 |
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Target: 5'- cGCUGC-ACUGCAUCGGucgcauCgaUC-CGCa -3' miRNA: 3'- -CGACGuUGACGUGGUCu-----GgaAGuGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 69991 | 0.66 | 0.876967 |
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Target: 5'- cGCUcGCGAUguaCACCAuucGACUUgaUCACGCg -3' miRNA: 3'- -CGA-CGUUGac-GUGGU---CUGGA--AGUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 17651 | 0.66 | 0.876967 |
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Target: 5'- aCUGCGGCUGgauaaCACCAG-CCUgCACa- -3' miRNA: 3'- cGACGUUGAC-----GUGGUCuGGAaGUGcg -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 15441 | 0.66 | 0.876967 |
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Target: 5'- --gGCAuaugGCACCAGACCaugaggUCuCGCg -3' miRNA: 3'- cgaCGUuga-CGUGGUCUGGa-----AGuGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 3351 | 0.66 | 0.869077 |
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Target: 5'- uGgUGCAGaucguaGUACCAGuucuuuCCUUCugGCg -3' miRNA: 3'- -CgACGUUga----CGUGGUCu-----GGAAGugCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 62973 | 0.66 | 0.899079 |
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Target: 5'- --aGCcGC-GCACCGGuACCgggaaaUCACGCg -3' miRNA: 3'- cgaCGuUGaCGUGGUC-UGGa-----AGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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