Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20795 | 5' | -52.6 | NC_004689.1 | + | 17651 | 0.66 | 0.876967 |
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Target: 5'- aCUGCGGCUGgauaaCACCAG-CCUgCACa- -3' miRNA: 3'- cGACGUUGAC-----GUGGUCuGGAaGUGcg -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 15441 | 0.66 | 0.876967 |
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Target: 5'- --gGCAuaugGCACCAGACCaugaggUCuCGCg -3' miRNA: 3'- cgaCGUuga-CGUGGUCUGGa-----AGuGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 3351 | 0.66 | 0.869077 |
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Target: 5'- uGgUGCAGaucguaGUACCAGuucuuuCCUUCugGCg -3' miRNA: 3'- -CgACGUUga----CGUGGUCu-----GGAAGugCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 44887 | 0.67 | 0.860938 |
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Target: 5'- cGCUGCGgaGCuUGCGCggauCGGGCCUgccggUCACcGCu -3' miRNA: 3'- -CGACGU--UG-ACGUG----GUCUGGA-----AGUG-CG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 51341 | 0.67 | 0.86011 |
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Target: 5'- cCUGCGaaACUGCaaaccccGCCAgGGCUUUC-CGCa -3' miRNA: 3'- cGACGU--UGACG-------UGGU-CUGGAAGuGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 11064 | 0.67 | 0.847415 |
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Target: 5'- uGCUGCGgguuguucggaucggGCUGCugCucgGGACCUuUCGaaaGCa -3' miRNA: 3'- -CGACGU---------------UGACGugG---UCUGGA-AGUg--CG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 12409 | 0.67 | 0.843941 |
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Target: 5'- cGUUGUAcGCcugGUugCGGAUgUUCGCGCg -3' miRNA: 3'- -CGACGU-UGa--CGugGUCUGgAAGUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 26301 | 0.67 | 0.843941 |
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Target: 5'- aGUUGaa--UGCGCCuucGACCUUgACGCc -3' miRNA: 3'- -CGACguugACGUGGu--CUGGAAgUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 32887 | 1 | 0.009229 |
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Target: 5'- gGCUGCAACUGCA-CAGACCUUCACGCu -3' miRNA: 3'- -CGACGUUGACGUgGUCUGGAAGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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