Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20795 | 5' | -52.6 | NC_004689.1 | + | 21054 | 0.71 | 0.638361 |
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Target: 5'- aGCUGCcucguACUcagagguuugaaGCAagcCCAGACCUUCaaGCGCg -3' miRNA: 3'- -CGACGu----UGA------------CGU---GGUCUGGAAG--UGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 48918 | 0.71 | 0.616209 |
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Target: 5'- aCUGCGACUGCGCgagCAGGgCUUCG-GCc -3' miRNA: 3'- cGACGUUGACGUG---GUCUgGAAGUgCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 15047 | 0.72 | 0.565594 |
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Target: 5'- gGCUaGCAgaccggaccucGCUGCACCAGuCCUaucgguccauggggCACGCa -3' miRNA: 3'- -CGA-CGU-----------UGACGUGGUCuGGAa-------------GUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 51815 | 0.74 | 0.466461 |
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Target: 5'- cCUGCAACcaCGCCAGACCcUCGuCGCu -3' miRNA: 3'- cGACGUUGacGUGGUCUGGaAGU-GCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 28425 | 0.74 | 0.436707 |
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Target: 5'- cCUGCAACUGCGucggCAGACCUUCGacccacggaaucUGCa -3' miRNA: 3'- cGACGUUGACGUg---GUCUGGAAGU------------GCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 16927 | 0.75 | 0.427034 |
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Target: 5'- aGCgGCGACUGCACCGGAU--UCGCu- -3' miRNA: 3'- -CGaCGUUGACGUGGUCUGgaAGUGcg -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 22718 | 0.77 | 0.313751 |
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Target: 5'- cGCUaugGCAucccaguCUGCACCGG-CCUUCAUGCc -3' miRNA: 3'- -CGA---CGUu------GACGUGGUCuGGAAGUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 22868 | 0.78 | 0.262842 |
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Target: 5'- gGCUGCGAuCUGUuguuccuucgacGCCAGAUCggCACGCg -3' miRNA: 3'- -CGACGUU-GACG------------UGGUCUGGaaGUGCG- -5' |
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| 20795 | 5' | -52.6 | NC_004689.1 | + | 32887 | 1 | 0.009229 |
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Target: 5'- gGCUGCAACUGCA-CAGACCUUCACGCu -3' miRNA: 3'- -CGACGUUGACGUgGUCUGGAAGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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